State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
Department of Medical Experimental Center, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, 266035, China.
Chembiochem. 2023 May 2;24(9):e202200778. doi: 10.1002/cbic.202200778. Epub 2023 Apr 12.
Glycosylation in live cell and animal modulate a constellation of biological functions. The advent of Chemical Biology has revolutionized the analysis and tailoring of glycans, by introducing myriads of glycan engineering methods. However, the ideal scenario to achieve glycan monitoring and structural manipulation at any hierarchical levels is unmet yet. Herein we review recent advances in the methodological innovation and the versatile applications of the protein-specific glycan visualization and editing in deciphering the biological functions of glycans. An outlook for future directions toward specific sugar-chain editing is also included.
糖基化在活细胞和动物中调节着一系列的生物学功能。化学生物学的出现通过引入无数的聚糖工程方法，彻底改变了聚糖的分析和定制。然而，在任何层次上实现聚糖监测和结构操作的理想情况尚未实现。本文综述了蛋白质特异性聚糖可视化和编辑方法的创新及其在破译聚糖生物学功能方面的多种应用的最新进展。还包括对特定糖链编辑的未来方向的展望。
