Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
One-Stop Specialty Center, Seoul National University Dental Hospital, Seoul, Republic of Korea.
J Periodontol. 2023 Aug;94(8):1032-1044. doi: 10.1002/JPER.22-0586. Epub 2023 Mar 12.
Guided bone regeneration (GBR) is the most widely used technique for overcoming the deficiency of alveolar bone. However, the progression of peri-implantitis in regenerative and pristine bone sites has not been fully investigated. The aim of this study is to compare experimental peri-implantitis around implants placed in pristine bone and GBR sites.
Bilateral mandibular first molars were extracted from six beagle dogs, and standardized horizontal ridge defect was simultaneously created at predetermined site in unilateral mandible. After 8 weeks, guided bone regeneration procedure was conducted at the defect site. After 16 weeks, implants (ϕ 3.6×8.0 mm) were placed at both extracted sites. This study included 3 months of active breakdown and another 3 months of spontaneous progression period. Radiographs were taken at each phase and specimens were obtained for histological, immunohistochemical, and polarized light microscopic analysis.
Marginal bone loss around implant did not show the significant differences between pristine bone and GBR sites during spontaneous progression period. In immunohistochemical analysis, inflammatory and immune-related cells were predominantly detected in peri-implantitis-affected area rather than unaffected area. In the polarized light microscopic analysis, substantial reductions in the amount and thickness of collagen fibers were observed in peri-implantitis-affected area compared with unaffected tissues. However, there were no significant differences in histological, immunohistochemical, polarized light microscopic outcomes between pristine bone and GBR sites.
Previous hard tissue grafting at the implant sites did not affect experimental peri-implantitis and exhibited similar radiographic, histological, immunohistochemical, and polarized light microscopic outcomes compared with those of pristine bone sites.
引导骨再生（GBR）是克服牙槽骨不足最常用的技术。然而，再生和原始骨部位种植体周围炎的进展尚未得到充分研究。本研究旨在比较种植体周围炎在原始骨和 GBR 部位的实验进展。
从 6 只比格犬中提取双侧下颌第一磨牙，并在单侧下颌预定部位同时创建标准化的水平牙槽嵴缺损。8 周后，在缺损部位进行引导骨再生。16 周后，在两个拔牙部位植入种植体（ϕ 3.6×8.0mm）。本研究包括 3 个月的主动破坏期和另外 3 个月的自发进展期。在每个阶段拍摄 X 光片，并获取标本进行组织学、免疫组织化学和偏光显微镜分析。
在自发进展期，种植体周围边缘骨丧失在原始骨和 GBR 部位之间没有显示出显著差异。在免疫组织化学分析中，炎症和免疫相关细胞主要在种植体周围炎受累区域而不是未受累区域检测到。在偏光显微镜分析中，与未受累组织相比，受累区域的胶原纤维数量和厚度明显减少。然而，在组织学、免疫组织化学、偏光显微镜结果方面，原始骨和 GBR 部位之间没有显著差异。
在种植部位进行的先前硬组织移植并没有影响实验性种植体周围炎，并且与原始骨部位相比，在影像学、组织学、免疫组织化学和偏光显微镜方面表现出相似的结果。
