Zhao Ning, Zhu Li, Liu Meichen, He Liangheng, Xu Hanhong, Jia Jinliang
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
J Agric Food Chem. 2023 Mar 1;71(8):3790-3799. doi: 10.1021/acs.jafc.2c07466. Epub 2023 Feb 17.
Intelligently responsive nanoparticles can improve insecticidal activity against target organisms and reduce the use of pesticides in agriculture. In this study, enzymatic hydrolysis lignin (EHL) nanocarriers with enzyme responsiveness were successfully prepared by electrostatic interaction, and abamectin (Abm)-loaded EHL-based nanoparticles (Abm@L-CL) were investigated. The release behavior of Abm@L-CL nanoparticles showed that Abm was released rapidly in the presence of cellulase and pectinase but slowly under natural conditions. The insecticidal activity of Abm@L-CL treatment (LC = 0.68 μg/mL) against nematodes () was significantly more effective than that of original Abm treatment (LC = 1.32 μg/mL). The mortality rate of Abm@L-CL was more than 90% by applying the same dose of Abm after 12 h. The bioactivity of Abm@L-CL against root-knot nematodes was 1.7-fold greater than that of Abm. The result of fluorescence indicated that nanoparticles could enter the intestinal tract through the oral cavity of nematodes and achieve obvious gastric toxicity. Furthermore, the enzyme-controlled lignin-based Abm nanocarriers could penetrate the tomato root near the elongation zone. This study provided intelligent enzyme-responsive nanocarriers for efficient management of soil-borne diseases and pests in green agricultural inputs.
智能响应型纳米颗粒可以提高对目标生物的杀虫活性，并减少农业中农药的使用。在本研究中，通过静电相互作用成功制备了具有酶响应性的酶解木质素（EHL）纳米载体，并对载有阿维菌素（Abm）的基于EHL的纳米颗粒（Abm@L-CL）进行了研究。Abm@L-CL纳米颗粒的释放行为表明，Abm在纤维素酶和果胶酶存在下快速释放，但在自然条件下缓慢释放。Abm@L-CL处理对线虫（）的杀虫活性（LC = 0.68 μg/mL）明显高于原始Abm处理（LC = 1.32 μg/mL）。在12小时后施用相同剂量的Abm，Abm@L-CL的死亡率超过90%。Abm@L-CL对根结线虫的生物活性比Abm高1.7倍。荧光结果表明，纳米颗粒可以通过线虫的口腔进入肠道并实现明显的胃毒作用。此外，酶控木质素基Abm纳米载体可以穿透番茄根伸长区附近。本研究为绿色农业投入品中土壤病虫害的高效治理提供了智能酶响应型纳米载体。
