Fu Zhinan, Chen Kai, Li Li, Zhao Fang, Wang Yan, Wang Mingwei, Shen Yue, Cui Haixin, Liu Dianhua, Guo Xuhong
State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China.
Engineering Research Center of Materials Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Xinjiang 832000, China.
Nanomaterials (Basel). 2018 Jun 20;8(6):449. doi: 10.3390/nano8060449.
Southern root-knot nematode () is a biotrophic parasite, causing enormous loss in global crop production annually. Abamectin (Abm) is a biological and high-efficiency pesticide against . In this study, a powerful method, flash nanoprecipitation (FNP), was adopted to successfully produce Abm-loaded nanoparticle suspensions with high drug loading capacity (>40%) and encapsulation efficiency (>95%), where amphiphilic block copolymers (BCPs) poly(lactic-co-glycolic acid)--poly(ethylene glycol) (PLGA--PEG), poly(d,l-lactide)--poly(ethylene glycol) (PLA--PEG), or poly(caprolactone)--poly(ethylene glycol) (PCL--PEG) were used as the stabilizer to prevent the nanoparticles from aggregation. The effect of the drug-to-stabilizer feed ratio on the particle stability were investigated. Moreover, the effect of the BCP composition on the morphology of Abm-loaded nanoparticles for controlling were discussed. Notably, spindle-like nanoparticles were obtained with PCL--PEG as the stabilizer and found significantly more efficient (98.4% mortality at 1 ppm particle concentration) than spherical nanoparticles using PLGA--PEG or PLA--PEG as the stabilizer. This work provides a more rapid and powerful method to prepare stable Abm-loaded nanoparticles with tunable morphologies and improved effectiveness for controlling .
南方根结线虫是一种活体营养型寄生虫，每年在全球作物生产中造成巨大损失。阿维菌素（Abm）是一种防治南方根结线虫的生物高效农药。在本研究中，采用了一种强大的方法——快速纳米沉淀法（FNP），成功制备了载药量高（>40%）、包封率高（>95%）的载阿维菌素纳米颗粒悬浮液，其中两亲性嵌段共聚物（BCP）聚乳酸-乙醇酸-聚乙二醇（PLGA-PEG）、聚（d，l-丙交酯）-聚乙二醇（PLA-PEG）或聚己内酯-聚乙二醇（PCL-PEG）用作稳定剂以防止纳米颗粒聚集。研究了药物与稳定剂进料比对颗粒稳定性的影响。此外，还讨论了BCP组成对用于防治南方根结线虫的载阿维菌素纳米颗粒形态的影响。值得注意的是，以PCL-PEG作为稳定剂获得了纺锤状纳米颗粒，并且发现其比使用PLGA-PEG或PLA-PEG作为稳定剂的球形纳米颗粒效率显著更高（在1 ppm颗粒浓度下死亡率为98.4%）。这项工作提供了一种更快速、强大的方法来制备具有可调形态且防治南方根结线虫效果更佳的稳定载阿维菌素纳米颗粒。
