Fredelius L, Johansson B, Bagger-Sjöbäck D, Wersäll J
Dept. of Otolaryngology, Karolinska Hospital, Stockholm, Sweden.
Hear Res. 1987;30(2-3):157-67. doi: 10.1016/0378-5955(87)90133-x.
A direct relationship between delivered sound energy and morphological overstimulation has been formulated in the so-called equal energy principle. The validity of this principle was systematically tested by studying the effects of acoustic overstimulation on the guinea pig cochlea by means of scanning electron microscopy (SEM) and light microscopy (LM) techniques. The animals in the present study were exposed to 3.85 kHz pure tones of various durations and intensities and allowed a recovery period of 4 weeks before the morphological changes in the cochlea were assessed. A 4-grade scale was devised in order to achieve a qualitative as well as a quantitative SEM evaluation of the cochlear damage. The results of the examinations by LM and SEM were compared and analysed statistically. No significant difference was observed between groups of animals exposed to a sound energy of 9.4 and 37.5 Pa2.h, irrespective of sound intensity (114 or 120 dB SPL). Groups of animals exposed to a higher energy level (150 Pa2.h) differed significantly from those exposed to higher and also lower sound energy levels. Groups exposed to different intensities (108, 114 and 120 dB SPL) did not differ significantly within this energy level.
在所谓的等能量原理中，已阐述了所传递的声能与形态学过度刺激之间的直接关系。通过扫描电子显微镜（SEM）和光学显微镜（LM）技术研究声过度刺激对豚鼠耳蜗的影响，系统地检验了该原理的有效性。本研究中的动物暴露于不同持续时间和强度的3.85kHz纯音下，并在评估耳蜗的形态学变化之前给予4周的恢复期。设计了一个4级量表，以便对耳蜗损伤进行定性和定量的SEM评估。对LM和SEM检查的结果进行了比较并进行统计学分析。暴露于9.4和37.5Pa²·h声能的动物组之间未观察到显著差异，无论声强如何（114或120dB SPL）。暴露于更高能量水平（150Pa²·h）的动物组与暴露于更高和更低声能水平的动物组有显著差异。在该能量水平内，暴露于不同强度（108、114和120dB SPL）的组之间没有显著差异。
