Vanderslice J T, Huang M H
U.S. Department of Agriculture, Beltsville Human Nutrition Center, MD 20705.
J Assoc Off Anal Chem. 1987 Sep-Oct;70(5):920-2.
Two extraction and liquid chromatographic procedures are presented which separate amprolium from compounds in poultry feed or premixes that could interfere with its fluorometric determination. The procedures are based on earlier work on the determination of thiamine in food samples. Amprolium is extracted from feed with a hexane-aqueous sulfosalicylic acid mix, separated on a C18 column, and detected fluorometrically after postcolumn derivatization. For premixes, water extraction is used. Values for the amprolium content of poultry feed obtained with these procedures are in good agreement with those obtained with AOAC official methods. It is suggested that these methods with suitable modifications may be of use for routine analysis of amprolium in feeds. The overall methods are rapid and appear to give reasonable results.
本文介绍了两种萃取和液相色谱方法，可将氨丙啉与家禽饲料或预混料中可能干扰其荧光测定的化合物分离。这些方法基于早期食品样品中硫胺素测定的研究。氨丙啉用己烷 - 磺基水杨酸水溶液混合物从饲料中萃取，在C18柱上分离，并在柱后衍生化后进行荧光检测。对于预混料，则采用水萃取法。用这些方法测得的家禽饲料中氨丙啉含量值与用美国官方分析化学师协会（AOAC）官方方法测得的值高度一致。建议对这些方法进行适当修改后，可用于饲料中氨丙啉的常规分析。总体而言，这些方法快速，且似乎能给出合理的结果。
