高效液相色谱法测定药用饲料和预混料中的磺胺硝苯和地恩硝唑。

High pressure liquid chromatographic determination of sulfanitran and dinsed in medicated feeds and premixes.

作者信息

Eaves K L, Colvin B M, Hanks A R, Bushway R J

出版信息

J Assoc Off Anal Chem. 1977 Sep;60(5):1064-6.

Abstract

A simple reverse phase high pressure liquid chromatographic method is described for determining sulfanitran (acetyl-p-nitrophenylsulfanilamide) and Dinsed (dinitrodiphenylsulfonylethylenediamine) in a variety of feed premixes and formulations. Feed premixes are extracted with dimethylformamide, and formulated feeds are extracted with hot methanol. The extract is filtered through medium porosity paper and injected into a liquid chromatograph equipped with a 254 nm ultraviolet detector and a 30 cm column packed with muBondapak C18. The mobile phase is acetonitrile-water (45 + 55) at a flow rate of 1.0 ml/min. Chromatography was complete in 10 min and peak heights were used for quantitation. Comparison of analyses of commercial samples by this method and by the AOAC colorimetric method, 42.176-42.179, showed close agreement. Recovery of spiked feed samples ranged from 98 to 105%. Butynorate and roxarsone, 2 other drugs which are normally found in combination with sulfanitran and Dinsed, do not interfere.

摘要

本文描述了一种简单的反相高效液相色谱法，用于测定各种饲料预混物和制剂中的磺胺硝苯（乙酰对硝基苯磺胺）和丁硒胺（二硝基二苯磺酰乙二胺）。饲料预混物用二甲基甲酰胺萃取，配制饲料用热甲醇萃取。萃取液通过中孔滤纸过滤，然后注入配备有254nm紫外检测器和填充μBondapak C18的30cm柱的液相色谱仪中。流动相为乙腈 - 水（45 + 55），流速为1.0ml/min。10分钟内完成色谱分析，峰高用于定量。用该方法与AOAC比色法（42.176 - 42.179）对商业样品进行分析比较，结果显示吻合度高。加标饲料样品的回收率在98%至105%之间。丁炔诺酯和洛克沙胂这两种通常与磺胺硝苯和丁硒胺同时存在的其他药物不产生干扰。