基于超精密弱测量的界面生物传感器。

Ultra-precise weak measurement-based interfacial biosensors.

作者信息

Zhang Lizhong, Huang Qiang, Zhang Xiaonan, Zeng Zhen, Zhang Hailong, Guan Tian, Xu Yang, Zhou Chongqi, Meng Lingqin, Liang Gengyu, Li Zhangyan, Wang Bei, Liu Le, Guo Cuixia, He Yonghong

机构信息

Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China; Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.

Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China; Shenzhen Shengqiang Technology Co., Ltd, China; Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.

出版信息

Talanta. 2023 May 15;257:124217. doi: 10.1016/j.talanta.2022.124217. Epub 2022 Dec 22.

DOI:10.1016/j.talanta.2022.124217

PMID:36801563

Abstract

In this study, an interfacial biosensing scheme with ultra-precision is proposed. The scheme uses weak measurement techniques to ensure ultra-high sensitivity of the sensing system while improving the stability of the system through self-referencing and pixel point averaging, thus achieving ultra-high detection accuracy of biological samples. In specific experiments, we have used the biosensor in this study to perform specific binding reaction experiments for protein A and Mouse IgG with a detection line of 2.71 ng/mL for IgG. In addition, the sensor is non-coated, simple in structure, easy to operate, and low in cost of use.

摘要

本研究提出了一种具有超高精度的界面生物传感方案。该方案采用弱测量技术，以确保传感系统的超高灵敏度，同时通过自参考和像素点平均提高系统的稳定性，从而实现生物样品的超高检测精度。在具体实验中，我们使用本研究中的生物传感器对蛋白A和小鼠IgG进行特异性结合反应实验，IgG的检测限为2.71 ng/mL。此外，该传感器无需涂层，结构简单，易于操作，使用成本低。