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大肠杆菌K-12 F'lac接合中受体菌的感受态

Recipient competence in F'lac matings of Escherichia coli K-12.

作者信息

Cullum J, Broda P

出版信息

J Bacteriol. 1979 Jan;137(1):281-4. doi: 10.1128/jb.137.1.281-284.1979.

Abstract

We studied recipient mating ability in the presence of excess F'lac donors. Ninety-five percent of recipients were able to receive F'lac in 30-min matings. Competition between an F'-lac donor and an F'lac traI donor, which mobilized a ColE1 derivative (pML2), showed that each recipient mated with an average of two to three donors in 30 min. Experiments in which the competing donor was added at different times showed that some competition occurred throughout the 30-min mating period, which suggested that aggregate formation was spread over this time.

摘要

我们研究了在存在过量F'lac供体的情况下受体的交配能力。95%的受体能够在30分钟的交配中接受F'lac。一个F'-lac供体与一个能动员ColE1衍生物(pML2)的F'lac traI供体之间的竞争表明,每个受体在30分钟内平均与两到三个供体交配。在不同时间添加竞争供体的实验表明,在整个30分钟的交配期都发生了一些竞争,这表明聚集体的形成在这段时间内是分散的。

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