[F'lac质粒从大肠杆菌K-12向欧文氏菌属细菌的转移]
[Transmission of F'lac plasmid from Escherichia coli K-12 to bacteria of the genus Erwinia].
作者信息
Prokulevich V A, Fomichev Iu K
出版信息
Genetika. 1978 Nov;14(11):1892-9.
The F'lac plasmid was transferred by conjugation from Escherichia coli K-12 W1655 to 21 lac- strains of Erwinia spp. (5.2 . 10(-6) to 6.8 . 10(-2) lac+ exconjugants per donor cell). Erw. herbicola and Erw. chrysanthemi were the better recipients than others. The degree of the stability of lac+ genes in Erwinia exconjugants depends on the strains. Stable exconjugants of Erwinia, which harbored F'lac plasmid, were able to utilize lactose, to transfer lac genes by conjugation to Erwinia spp. and E. coli, and were sensitive to the F-specific phages f1, f2, Qbeta. The F'lac plasmid was eliminated from the exconjugants by the treatment with acridine orange, which indicates that this genetic element is not integrated into the Erwinia chromosome.
F'lac 质粒通过接合作用从大肠杆菌 K-12 W1655 转移至 21 株欧文氏菌属的 lac- 菌株（每个供体细胞产生 5.2×10⁻⁶ 至 6.8×10⁻² 个 lac⁺ 接合子）。草生欧文氏菌和菊欧文氏菌比其他菌株更易成为受体菌。欧文氏菌接合子中 lac⁺ 基因的稳定程度取决于菌株。携带 F'lac 质粒的欧文氏菌稳定接合子能够利用乳糖，通过接合作用将 lac 基因转移至欧文氏菌属和大肠杆菌，并且对 F 特异性噬菌体 f1、f2、Qβ 敏感。用吖啶橙处理可使接合子中的 F'lac 质粒消除，这表明该遗传元件未整合到欧文氏菌染色体中。
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