Lu Yi, Zhang Zhaoxue, Wang Yingyang, Peng Fangyuan, Yang Zhaoguang, Li Haipu
Center for Environment and Water Resources, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China; Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Changsha, 410083, China.
Center for Environment and Water Resources, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China; Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Changsha, 410083, China; Key Laboratory of Testing and Tracing of Rare Earth Products for State Market Regulation, Jiangxi University of Science and Technology, Ganzhou, 341000, China.
J Environ Manage. 2023 May 15;334:117504. doi: 10.1016/j.jenvman.2023.117504. Epub 2023 Feb 15.
Boehmeria nivea L. (ramie) is a promising phytoremediation plant for antimony (Sb)-contaminated soils. However, the uptake, tolerance, and detoxification mechanisms of ramie to Sb, which are the basis for finding efficient phytoremediation strategies, remain unclear. In the present study, ramie was exposed to 0, 1, 10, 50, 100, and 200 mg/L of antimonite (Sb(III)) or antimonate (Sb(V)) for 14 days in hydroponic culture. The Sb concentration, speciation, subcellular distribution, and antioxidant and ionomic responses in ramie were investigated. The results illustrated that ramie was more effective in the uptake of Sb(III) than Sb(V). Most of the Sb accumulated in ramie roots, with the highest level reaching 7883.58 mg/kg. Sb(V) was the predominant species in leaves, with 80.77-96.38% and 100% in the Sb(III) and Sb(V) treatments, respectively. Immobilization of Sb on the cell wall and leaf cytosol was the primary mechanism of accumulation. Superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) contributed significantly to root defense against Sb(III), while CAT and glutathione peroxidase (GPX) were the major antioxidants in leaves. CAT and POD played crucial roles in the defense against Sb(V). B, Ca, K, Mg, and Mn in Sb(V)-treated leaves and K and Cu in Sb(III)-treated leaves may be related to the biological processes of Sb toxicity mitigation. This study is the first to investigate the ionomic responses of plants toward Sb and could provide valuable information for the phytoremediation of Sb-polluted soils.
苎麻是一种用于锑污染土壤修复的极具潜力的植物。然而，苎麻对锑的吸收、耐受和解毒机制尚不清楚，而这些机制是寻找高效植物修复策略的基础。在本研究中，苎麻在水培条件下分别暴露于0、1、10、50、100和200mg/L的亚锑酸盐（Sb(III)）或锑酸盐（Sb(V)）中14天。研究了苎麻中锑的浓度、形态、亚细胞分布以及抗氧化和离子组学响应。结果表明，苎麻对Sb(III)的吸收比对Sb(V)更有效。大部分锑积累在苎麻根部，最高含量达到7883.58mg/kg。Sb(V)是叶片中的主要形态，在Sb(III)和Sb(V)处理中分别占80.77 - 96.38%和100%。锑在细胞壁和叶细胞质中的固定是积累的主要机制。超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）对根部抵御Sb(III)有显著作用，而CAT和谷胱甘肽过氧化物酶（GPX）是叶片中的主要抗氧化剂。CAT和POD在抵御Sb(V)中起关键作用。Sb(V)处理叶片中的硼、钙、钾、镁和锰以及Sb(III)处理叶片中的钾和铜可能与减轻锑毒性的生物学过程有关。本研究首次调查了植物对锑的离子组学响应，可为锑污染土壤的植物修复提供有价值的信息。
