Center for Environment and Water Resources, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China; Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Changsha, 410083, China.
Center for Environment and Water Resources, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China; Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Changsha, 410083, China.
J Environ Manage. 2023 Oct 1;343:118195. doi: 10.1016/j.jenvman.2023.118195. Epub 2023 May 23.
Soil antimony (Sb) pollution is a global concern that threatens food security and human health. Boehmeria nivea L. (ramie) is a promising phytoremediation plant exhibiting high tolerance and enrichment capacity for Sb. To reveal the molecular mechanisms and thus enhance the ramie uptake, transport, and detoxification of Sb with practical strategies, a hydroponic experiment was conducted to compare the physiological and transcriptomic responses of ramie towards antimonite (Sb(Ⅲ)) and antimonate (Sb(Ⅴ)). Phenotypic results showed that Sb(Ⅲ) had a stronger inhibitory effect on the growth of ramie. Root Sb content under Sb(Ⅲ) was 2.43 times higher than that in Sb(Ⅴ) treatment. Based on the ribonucleic acid sequencing (RNA-Seq) technique, 3915 and 999 significant differentially expressed genes (DEGs) were identified under Sb(Ⅲ) and Sb(Ⅴ), respectively. Transcriptomic analysis revealed that ramie showed different adaptation strategies to Sb(Ⅲ) and Sb(V). Key DEGs and their involved pathways such as catalytic activity, carbohydrate metabolisms, phenylpropanoid biosynthesis, and cell wall modification were identified to perform crucial roles in Sb tolerance and detoxification. Two heavy metal-associated domain-type genes, six heavy metal-associated isoprenylated plant proteins, and nine ABC transporters showed possible roles in the transport and detoxification of Sb. The significant upregulation of NRAMP5 and three NIPs suggested their roles in the transport of Sb(V). This study is the basis for future research to identify the exact genes and biological processes that can effectively enhance Sb accumulation or improve plant tolerance to Sb, thereby promoting the phytoremediation of Sb-polluted soils.
土壤中的锑污染是一个全球性的问题，它威胁着食品安全和人类健康。苎麻是一种很有前途的植物修复植物，对锑具有较高的耐受性和富集能力。为了揭示分子机制，从而用实际策略增强苎麻对锑的吸收、转运和解毒，我们进行了水培实验，比较了苎麻对亚锑酸盐（Sb(Ⅲ)）和锑酸盐（Sb(Ⅴ)）的生理和转录组响应。表型结果表明，Sb(Ⅲ)对苎麻的生长抑制作用更强。Sb(Ⅲ)处理下根中的 Sb 含量是 Sb(Ⅴ)处理下的 2.43 倍。基于核糖核酸测序（RNA-Seq）技术，分别在 Sb(Ⅲ)和 Sb(Ⅴ)下鉴定出 3915 个和 999 个显著差异表达基因（DEGs）。转录组分析表明，苎麻对 Sb(Ⅲ)和 Sb(V)表现出不同的适应策略。关键 DEGs 及其涉及的途径，如催化活性、碳水化合物代谢、苯丙烷生物合成和细胞壁修饰，被认为在 Sb 耐受和解毒中发挥着关键作用。两个重金属相关结构域型基因、六个重金属相关异戊二烯基植物蛋白和九个 ABC 转运体可能在 Sb 的转运和解毒中发挥作用。NRAMP5 和三个 NIPs 的显著上调表明它们在 Sb(V)的转运中发挥作用。这项研究为未来的研究提供了基础，可以确定有效增强 Sb 积累或提高植物对 Sb 耐受性的特定基因和生物学过程，从而促进 Sb 污染土壤的植物修复。
