Kumari Mamta, Gupta Rajendar P, Bagri Preeti, Singh Renu
Department of Veterinary Pathology, College of Veterinary Science, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India.
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India.
Vet Immunol Immunopathol. 2023 Apr;258:110562. doi: 10.1016/j.vetimm.2023.110562. Epub 2023 Feb 10.
Escherichia coli infection is the major bacterial disease affecting the poultry industry and the continuous use of antibiotics in poultry farming has resulted in antibiotic resistance. So this study was planned to evaluate the use of an ecologically safe alternative to fight against infections. The leaf gel of the Aloe vera plant was selected based on its antibacterial activity assessed in in-vitro tests. The objective of the present study was to evaluate the effect of A. vera leaf extract supplementation on the severity of clinical signs and pathological lesions, mortality rate, levels of antioxidant enzymes, and immune response in experimentally E. coli-infected broiler chicks. Broiler chicks were supplemented with aqueous Aloe vera Leaf (AVL) extract @ 20 ml per liter of water from day one of age. After seven days of age, they were experimentally infected with E. coli O78 @ 10 CFU/0.5 ml intraperitoneally. Blood was collected at weekly intervals up to 28 days and assayed for antioxidant enzyme assays, and humoral and cellular immune response. The birds were observed daily for clinical signs and mortality. Dead birds were examined for gross lesions and representative tissues were processed for histopathology. The activities of antioxidants, Glutathione reductase (GR), and Glutathione-S-Transferase (GST) were significantly higher than the control infected group. The E. coli-specific antibody titer and Lymphocyte stimulation Index were comparatively higher in the AVL extract-supplemented infected group as compared to the control infected group. No considerable change was noted in the severity of clinical signs and pathological lesions, and mortality. Thus, Aloe vera Leaf gel extract enhanced the antioxidant activities and cellular immune responses of infected broiler chicks to combat this infection.
大肠杆菌感染是影响家禽业的主要细菌性疾病，家禽养殖中抗生素的持续使用已导致抗生素耐药性。因此，本研究旨在评估使用一种生态安全的替代物来对抗感染。基于其在体外试验中评估的抗菌活性，选择了芦荟植物的叶凝胶。本研究的目的是评估补充芦荟叶提取物对实验性感染大肠杆菌的肉鸡雏鸡临床症状严重程度、病理损伤、死亡率、抗氧化酶水平和免疫反应的影响。从1日龄开始，给肉鸡雏鸡补充每升水20毫升的芦荟叶水提取物。7日龄后，它们通过腹腔注射10 CFU/0.5毫升的大肠杆菌O78进行实验性感染。每隔一周采集血液，直至28天，进行抗氧化酶检测以及体液和细胞免疫反应检测。每天观察鸡的临床症状和死亡率。对死亡鸡进行大体病变检查，并对代表性组织进行组织病理学处理。抗氧化剂、谷胱甘肽还原酶（GR）和谷胱甘肽 - S - 转移酶（GST）的活性显著高于感染对照组。与感染对照组相比，补充芦荟叶提取物的感染组中大肠杆菌特异性抗体滴度和淋巴细胞刺激指数相对较高。临床症状严重程度、病理损伤和死亡率没有明显变化。因此，芦荟叶凝胶提取物增强了感染肉鸡雏鸡的抗氧化活性和细胞免疫反应，以对抗这种感染。
