Zamir L O, Devor K A
Université du Québec, Institut Armand-Frappier, Laval-des-Rapides, Canada.
J Biol Chem. 1987 Nov 15;262(32):15348-53.
A kinetic pulse-labeling method was utilized in Fusarium culmorum to detect plausible biosynthetic intermediates and differentiate them from dead-end metabolites. The ultimate test to demonstrate a precursor relies on feeding experiments. We now report the detection of four new metabolites, one of them (compound 1) behaves as a dead-end metabolite, whereas compounds 2, 3, and 4 seem to be putative intermediates: they metabolize with time just when 3-acetyldeoxynivalenol (3-ADN) and/or sambucinol (SOL) start to be produced. Feeding experiments confirmed these results: compound 1 is not converted to 3-ADN or SOL, and compounds 2-4 are precursors to 3-ADN. In addition 3 is a precursor to SOL.
在禾谷镰刀菌中采用了一种动力学脉冲标记方法，以检测可能的生物合成中间体，并将它们与终产物代谢物区分开来。证明一种前体的最终测试依赖于饲喂实验。我们现在报告检测到四种新的代谢物，其中一种（化合物1）表现为终产物代谢物，而化合物2、3和4似乎是假定的中间体：就在3-乙酰脱氧雪腐镰刀菌烯醇（3-ADN）和/或山梨醇（SOL）开始产生时，它们会随着时间代谢。饲喂实验证实了这些结果：化合物1不会转化为3-ADN或SOL，化合物2-4是3-ADN的前体。此外，化合物3是SOL的前体。
