• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种类似中东呼吸综合征的蝙蝠冠状病毒在穿山甲中传播，并利用人类 DPP4 和宿主蛋白酶进入细胞。

A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry.

机构信息

CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.

CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China; University of Chinese Academy of Sciences, Beijing, China.

出版信息

Cell. 2023 Feb 16;186(4):850-863.e16. doi: 10.1016/j.cell.2023.01.019.

DOI:10.1016/j.cell.2023.01.019
PMID:36803605
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9933427/
Abstract

It is unknown whether pangolins, the most trafficked mammals, play a role in the zoonotic transmission of bat coronaviruses. We report the circulation of a novel MERS-like coronavirus in Malayan pangolins, named Manis javanica HKU4-related coronavirus (MjHKU4r-CoV). Among 86 animals, four tested positive by pan-CoV PCR, and seven tested seropositive (11 and 12.8%). Four nearly identical (99.9%) genome sequences were obtained, and one virus was isolated (MjHKU4r-CoV-1). This virus utilizes human dipeptidyl peptidase-4 (hDPP4) as a receptor and host proteases for cell infection, which is enhanced by a furin cleavage site that is absent in all known bat HKU4r-CoVs. The MjHKU4r-CoV-1 spike shows higher binding affinity for hDPP4, and MjHKU4r-CoV-1 has a wider host range than bat HKU4-CoV. MjHKU4r-CoV-1 is infectious and pathogenic in human airways and intestinal organs and in hDPP4-transgenic mice. Our study highlights the importance of pangolins as reservoir hosts of coronaviruses poised for human disease emergence.

摘要

目前尚不清楚穿山甲——最常被走私的哺乳动物——是否在蝙蝠冠状病毒的人畜共患病传播中发挥作用。我们报告了一种新型 MERS 样冠状病毒在马来穿山甲中的传播，命名为 Manis javanica HKU4 相关冠状病毒（MjHKU4r-CoV）。在 86 只动物中，有 4 只通过 pan-CoV PCR 检测呈阳性，7 只血清学检测呈阳性（11%和 12.8%）。我们获得了 4 个几乎完全相同（99.9%）的基因组序列，其中一个病毒被分离（MjHKU4r-CoV-1）。该病毒利用人二肽基肽酶-4（hDPP4）作为受体和宿主蛋白酶进行细胞感染，其增强作用依赖于一个弗林切割位点，而所有已知的蝙蝠 HKU4r-CoVs 中均不存在该位点。MjHKU4r-CoV-1 的刺突蛋白与人 DPP4 的结合亲和力更高，而 MjHKU4r-CoV-1 的宿主范围比蝙蝠 HKU4-CoV 更广。MjHKU4r-CoV-1 在人类气道和肠道器官以及 hDPP4 转基因小鼠中具有感染性和致病性。我们的研究强调了穿山甲作为冠状病毒潜在宿主在人类疾病出现中的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/8420332bf681/gr7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/aa289a9e3af9/fx1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/e25c810dc0b3/figs1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/736c8a3aa6c9/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/3d0259e50b1d/figs2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/aadc2c6a473d/figs3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/0865d1ea65a3/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/7a35ab4918b8/figs4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/b324516e0169/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/0545b02ccd80/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/3b4cf6b9d371/figs5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/b4aec0788c73/figs6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/6da379d9fdc7/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/11f330830030/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/86de857dd1a1/figs7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/8420332bf681/gr7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/aa289a9e3af9/fx1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/e25c810dc0b3/figs1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/736c8a3aa6c9/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/3d0259e50b1d/figs2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/aadc2c6a473d/figs3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/0865d1ea65a3/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/7a35ab4918b8/figs4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/b324516e0169/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/0545b02ccd80/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/3b4cf6b9d371/figs5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/b4aec0788c73/figs6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/6da379d9fdc7/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/11f330830030/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/86de857dd1a1/figs7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac5/9933427/8420332bf681/gr7_lrg.jpg

相似文献

1
A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry.一种类似中东呼吸综合征的蝙蝠冠状病毒在穿山甲中传播，并利用人类 DPP4 和宿主蛋白酶进入细胞。
Cell. 2023 Feb 16;186(4):850-863.e16. doi: 10.1016/j.cell.2023.01.019.
2
Pangolin HKU4-related coronaviruses found in greater bamboo bats from southern China.在中国南方的大竹蝠中发现了与穿山甲 HKU4 相关的冠状病毒。
Virol Sin. 2023 Dec;38(6):868-876. doi: 10.1016/j.virs.2023.11.003. Epub 2023 Nov 13.
3
Receptor usage and cell entry of bat coronavirus HKU4 provide insight into bat-to-human transmission of MERS coronavirus.蝙蝠冠状病毒HKU4的受体使用情况及细胞进入机制为中东呼吸综合征冠状病毒从蝙蝠向人类的传播提供了线索。
Proc Natl Acad Sci U S A. 2014 Aug 26;111(34):12516-21. doi: 10.1073/pnas.1405889111. Epub 2014 Aug 11.
4
Discovery of Novel Bat Coronaviruses in South China That Use the Same Receptor as Middle East Respiratory Syndrome Coronavirus.在中国南方发现使用与中东呼吸综合征冠状病毒相同受体的新型蝙蝠冠状病毒。
J Virol. 2018 Jun 13;92(13). doi: 10.1128/JVI.00116-18. Print 2018 Jul 1.
5
The tetraspanin CD9 facilitates MERS-coronavirus entry by scaffolding host cell receptors and proteases.四跨膜蛋白CD9通过搭建宿主细胞受体和蛋白酶促进中东呼吸综合征冠状病毒进入细胞。
PLoS Pathog. 2017 Jul 31;13(7):e1006546. doi: 10.1371/journal.ppat.1006546. eCollection 2017 Jul.
6
Bat origins of MERS-CoV supported by bat coronavirus HKU4 usage of human receptor CD26.蝙蝠冠状病毒HKU4对人类受体CD26的利用支持中东呼吸综合征冠状病毒起源于蝙蝠。
Cell Host Microbe. 2014 Sep 10;16(3):328-37. doi: 10.1016/j.chom.2014.08.009.
7
Isolation of MERS-related coronavirus from lesser bamboo bats that uses DPP4 and infects human-DPP4-transgenic mice.从使用 DPP4 的小型竹蝠中分离出与中东呼吸综合征相关的冠状病毒，并感染人源 DPP4 转基因小鼠。
Nat Commun. 2021 Jan 11;12(1):216. doi: 10.1038/s41467-020-20458-9.
8
Permissivity of Dipeptidyl Peptidase 4 Orthologs to Middle East Respiratory Syndrome Coronavirus Is Governed by Glycosylation and Other Complex Determinants.二肽基肽酶4直系同源物对中东呼吸综合征冠状病毒的易感性受糖基化和其他复杂决定因素的调控。
J Virol. 2017 Sep 12;91(19). doi: 10.1128/JVI.00534-17. Print 2017 Oct 1.
9
Isolation and characterization of a pangolin-borne HKU4-related coronavirus that potentially infects human-DPP4-transgenic mice.一种可能感染人二肽基肽酶4（DPP4）转基因小鼠的穿山甲携带的与HKU4相关冠状病毒的分离与鉴定
Nat Commun. 2024 Feb 5;15(1):1048. doi: 10.1038/s41467-024-45453-2.
10
Replication of MERS and SARS coronaviruses in bat cells offers insights to their ancestral origins.MERS 和 SARS 冠状病毒在蝙蝠细胞中的复制为它们的祖先起源提供了线索。
Emerg Microbes Infect. 2018 Dec 10;7(1):209. doi: 10.1038/s41426-018-0208-9.

引用本文的文献

1
Discovery and mechanistic insights of dibenzoylmethane as a broad spectrum inhibitor of coronavirus.二苯甲酰甲烷作为冠状病毒广谱抑制剂的发现及作用机制研究
PLoS Pathog. 2025 Sep 8;21(9):e1013492. doi: 10.1371/journal.ppat.1013492. eCollection 2025 Sep.
2
Characterization of a MERS-related betacoronavirus in Danish brown long-eared bats (Plecotus auritus).丹麦棕长耳蝠（Plecotus auritus）中一种与中东呼吸综合征相关的β冠状病毒的特征分析。
Virol J. 2025 Aug 18;22(1):283. doi: 10.1186/s12985-025-02883-8.
3
Cross-neutralization ability of anti-MERS-CoV monoclonal antibodies against a variety of merbecoviruses.

本文引用的文献

1
Trafficked Malayan pangolins contain viral pathogens of humans.被贩运的马来亚穿山甲携带人类病毒病原体。
Nat Microbiol. 2022 Aug;7(8):1259-1269. doi: 10.1038/s41564-022-01181-1. Epub 2022 Aug 2.
2
Ecological study of cave nectar bats reveals low risk of direct transmission of bat viruses to humans.洞穴花蜜蝙蝠的生态学研究表明，蝙蝠病毒直接传播给人类的风险较低。
Zool Res. 2022 Jul 18;43(4):514-522. doi: 10.24272/j.issn.2095-8137.2021.480.
3
Want to prevent pandemics? Stop spillovers.想要预防大流行？阻止病毒外溢。
抗中东呼吸综合征冠状病毒单克隆抗体对多种默贝病毒的交叉中和能力。
Front Microbiol. 2025 Jul 16;16:1593095. doi: 10.3389/fmicb.2025.1593095. eCollection 2025.
4
Merbecovirus S2 subunit vaccines elicit cross reactive antibodies and provide partial protection against MERS coronavirus.默贝科病毒S2亚基疫苗可引发交叉反应性抗体，并对中东呼吸综合征冠状病毒提供部分保护。
Npj Viruses. 2025 Jul 29;3(1):60. doi: 10.1038/s44298-025-00142-9.
5
Structural and functional constraints on spike activation and host protease utilization limit cell entry of SARS-CoV-2-related bat coronaviruses.刺突激活和宿主蛋白酶利用的结构与功能限制，制约了与SARS-CoV-2相关的蝙蝠冠状病毒的细胞进入。
J Virol. 2025 Jul 24:e0100725. doi: 10.1128/jvi.01007-25.
6
Structures and receptor binding activities of merbecovirus spike proteins reveal key signatures for human DPP4 adaptation.默贝科病毒刺突蛋白的结构与受体结合活性揭示了人类二肽基肽酶4适应性的关键特征。
Sci Adv. 2025 Jul 11;11(28):eadv7296. doi: 10.1126/sciadv.adv7296.
7
A single-dose intranasal immunization with a novel bat influenza A virus-vectored MERS vaccine provides effective protection against lethal MERS-CoV challenge.用一种新型甲型蝙蝠流感病毒载体中东呼吸综合征疫苗进行单剂量鼻内免疫可有效抵御致死性中东呼吸综合征冠状病毒攻击。
mBio. 2025 Jun 30:e0110725. doi: 10.1128/mbio.01107-25.
8
and characterization of a bat merbecovirus with ACE2- and DPP4-independent cell entry.以及一种具有不依赖ACE2和DPP4的细胞进入方式的蝙蝠美贝病毒的特征描述。
J Virol. 2025 Jul 22;99(7):e0072725. doi: 10.1128/jvi.00727-25. Epub 2025 Jun 17.
9
Exclusive: Inside the thriving wild-animal markets that could start the next pandemic.独家报道：探秘那些可能引发下一场大流行的繁荣野生动物市场。
Nature. 2025 Jun;642(8066):26-28. doi: 10.1038/d41586-025-01690-z.
10
ACE2 from Pipistrellus abramus bats is a receptor for HKU5 coronaviruses.来自棕果蝠的血管紧张素转换酶2是HKU5冠状病毒的受体。
Nat Commun. 2025 May 28;16(1):4932. doi: 10.1038/s41467-025-60286-3.
Nature. 2022 May;605(7910):419-422. doi: 10.1038/d41586-022-01312-y.
4
The high diversity of SARS-CoV-2-related coronaviruses in pangolins alerts potential ecological risks.穿山甲体内与严重急性呼吸综合征冠状病毒2（SARS-CoV-2）相关的冠状病毒的高度多样性警示了潜在的生态风险。
Zool Res. 2021 Nov 18;42(6):834-844. doi: 10.24272/j.issn.2095-8137.2021.334.
5
Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor.鉴定出一种使用蝙蝠 ACE2 受体的新型谱系蝙蝠 SARS 相关冠状病毒。
Emerg Microbes Infect. 2021 Dec;10(1):1507-1514. doi: 10.1080/22221751.2021.1956373.
6
The furin cleavage site in the SARS-CoV-2 spike protein is required for transmission in ferrets.SARS-CoV-2 刺突蛋白中的弗林裂解位点是在雪貂中传播所必需的。
Nat Microbiol. 2021 Jul;6(7):899-909. doi: 10.1038/s41564-021-00908-w. Epub 2021 Apr 27.
7
Bat and pangolin coronavirus spike glycoprotein structures provide insights into SARS-CoV-2 evolution.蝙蝠和穿山甲冠状病毒刺突糖蛋白结构为深入了解 SARS-CoV-2 进化提供了线索。
Nat Commun. 2021 Mar 11;12(1):1607. doi: 10.1038/s41467-021-21767-3.
8
Development of A MERS-CoV Replicon Cell Line for Antiviral Screening.中东呼吸综合征冠状病毒复制子细胞系的建立及其在抗病毒药物筛选中的应用。
Virol Sin. 2021 Aug;36(4):730-735. doi: 10.1007/s12250-020-00341-z. Epub 2021 Feb 22.
9
Evidence for SARS-CoV-2 related coronaviruses circulating in bats and pangolins in Southeast Asia.有证据表明，东南亚的蝙蝠和穿山甲中存在与 SARS-CoV-2 相关的冠状病毒。
Nat Commun. 2021 Feb 9;12(1):972. doi: 10.1038/s41467-021-21240-1.
10
Loss of furin cleavage site attenuates SARS-CoV-2 pathogenesis.去除furin 酶切位点可减轻 SARS-CoV-2 的发病机制。
Nature. 2021 Mar;591(7849):293-299. doi: 10.1038/s41586-021-03237-4. Epub 2021 Jan 25.