Graduate Program in Sciences and Biotechnology, Institute of Biology, Fluminense Federal University (UFF), R. Mario Santos Braga, S/n. Centro, Niterói, RJ, CEP 24.020-141, Brazil; Research Group of Experimental and Aquatic Ecology, Institute of Biosciences (IBIO), Federal University of the State of Rio de Janeiro (UNIRIO), Av. Pasteur, 458-307, Urca, Rio de Janeiro, RJ, CEP: 22.290-240, Brazil.
Graduate Program in Neotropical Biodiversity (PPGBIO), Institute of Biosciences (IBIO), Federal University of the State of Rio de Janeiro (UNIRIO), Av. Pasteur, 458, Urca, Rio de Janeiro, RJ, CEP: 22.290-255, Brazil; Research Group of Experimental and Aquatic Ecology, Institute of Biosciences (IBIO), Federal University of the State of Rio de Janeiro (UNIRIO), Av. Pasteur, 458-307, Urca, Rio de Janeiro, RJ, CEP: 22.290-240, Brazil.
Fish Shellfish Immunol. 2023 Mar;134:108622. doi: 10.1016/j.fsi.2023.108622. Epub 2023 Feb 17.
The brown mussel Perna perna is a valuable fishing resource, primarily in tropical and subtropical coastal regions. Because of their filter-feeding habits, mussels are directly exposed to bacteria in the water column. Escherichia coli (EC) and Salmonella enterica (SE) inhabit human guts and reach the marine environment through anthropogenic sources, such as sewage. Vibrio parahaemolyticus (VP) is indigenous to coastal ecosystems but can be harmful to shellfish. In this study, we aimed to assess the protein profile of the hepatopancreas of P. perna mussel challenged by introduced - E. coli and S. enterica - and indigenous marine bacteria - V. parahaemolyticus. Bacterial-challenge groups were compared with non-injected (NC) and injected control (IC) - that consisted in mussels not challenged and mussels injected with sterile PBS-NaCl, respectively. Through LC-MS/MS proteomic analysis, 3805 proteins were found in the hepatopancreas of P. perna. From the total, 597 were significantly different among conditions. Mussels injected with VP presented 343 proteins downregulated compared with all the other conditions, suggesting that VP suppresses their immune response. Particularly, 31 altered proteins - upregulated or downregulated - for one or more challenge groups (EC, SE, and VP) compared with controls (NC and IC) are discussed in detail in the paper. For the three tested bacteria, significantly different proteins were found to perform critical roles in immune response at all levels, namely: recognition and signal transduction; transcription; RNA processing; translation and protein processing; secretion; and humoral effectors. This is the first shotgun proteomic study in P. perna mussel, therefore providing an overview of the protein profile of the mussel hepatopancreas, focused on the immune response against bacteria. Hence, it is possible to understand the immune-bacteria relationship at molecular levels better. This knowledge can support the development of strategies and tools to be applied to coastal marine resource management and contribute to the sustainability of coastal systems.
贻贝是一种有价值的渔业资源，主要分布在热带和亚热带沿海地区。由于它们的滤食习性，贻贝直接暴露在水柱中的细菌中。大肠杆菌（EC）和沙门氏菌（SE）栖息在人类肠道中，并通过人类活动来源（如污水）进入海洋环境。副溶血性弧菌（VP）是沿海生态系统的本土生物，但对贝类有害。在这项研究中，我们旨在评估受引入的大肠杆菌和沙门氏菌以及本土海洋细菌副溶血性弧菌挑战的贻贝肝胰腺的蛋白质谱。细菌挑战组与未注射（NC）和注射对照（IC）组进行了比较，后者由未受挑战的贻贝和注射无菌 PBS-NaCl 的贻贝组成。通过 LC-MS/MS 蛋白质组学分析，在 P. perna 肝胰腺中发现了 3805 种蛋白质。在总数中，有 597 种在条件之间存在显著差异。与所有其他条件相比，注射 VP 的贻贝有 343 种蛋白质下调，表明 VP 抑制了它们的免疫反应。特别是，与对照（NC 和 IC）相比，对于一个或多个挑战组（EC、SE 和 VP）上调或下调的 31 种改变的蛋白质在本文中进行了详细讨论。对于测试的三种细菌，与对照相比，在所有水平上都发现具有显著差异的蛋白质在免疫反应中起关键作用，即：识别和信号转导；转录；RNA 处理；翻译和蛋白质加工；分泌；和体液效应物。这是 P. perna 贻贝中首次进行的鸟枪法蛋白质组学研究，因此提供了贻贝肝胰腺蛋白质谱的概述，重点是针对细菌的免疫反应。因此，可以更好地了解分子水平上的免疫-细菌关系。这些知识可以支持沿海海洋资源管理策略和工具的开发，并有助于沿海系统的可持续性。
