Okubo Y, Honma Y, Suzuki S
J Bacteriol. 1979 Jan;137(1):677-80. doi: 10.1128/jb.137.1.677-680.1979.
The mannans from Candida albicans strains NIH A-207 (serotype A), NIH B-792 (serotype B), and J-1012 (serotype C) were fractionated on a column of diethylaminoethyl-Sephadex into five subfractions containing different amounts of phosphate. Antibody-precipitating activities of the mannan subfractions of strains NIH A-207 and NIH B-792 were proportional to their phosphate content, while those of strain J-1012 did not show regularly proportional precipitin activity. A similar tendency was also observed in the cross-reaction between the mannan su,fractions of strains NIH A-207 and J-1012 and their heterologous antisera. The mannans of strain NIH B-792 showed lower cross-reactivities against antisera of strains NIH A-207 and NIH B-792, i.e., only two subfractions containing larger amounts of phosphate were able to react with these antisera.
将白色念珠菌菌株NIH A - 207(血清型A)、NIH B - 792(血清型B)和J - 1012(血清型C)的甘露聚糖在二乙氨基乙基 - 葡聚糖柱上进行分级分离,得到五个含有不同量磷酸盐的亚组分。菌株NIH A - 207和NIH B - 792的甘露聚糖亚组分的抗体沉淀活性与其磷酸盐含量成正比,而菌株J - 1012的抗体沉淀活性则没有呈现出规律的正比关系。在菌株NIH A - 207和J - 1012的甘露聚糖亚组分与其异源抗血清之间的交叉反应中也观察到了类似的趋势。菌株NIH B - 792的甘露聚糖对菌株NIH A - 207和NIH B - 792的抗血清显示出较低的交叉反应性,即只有两个含有较多磷酸盐的亚组分能够与这些抗血清发生反应。