鉴定质粒编码蛋白的简易方法。
Simple method for identification of plasmid-coded proteins.
作者信息
Sancar A, Hack A M, Rupp W D
出版信息
J Bacteriol. 1979 Jan;137(1):692-3. doi: 10.1128/jb.137.1.692-693.1979.
Abstract
Proteins encoded by plasmid DNA are specifically labeled in UV-irradiated cells of Escherichia coli carrying recA and uvrA mutations because extensive degradation of the chromosome DNA occurs concurrently with amplification of plasmid DNA.
摘要
在携带recA和uvrA突变的大肠杆菌经紫外线照射的细胞中，质粒DNA编码的蛋白质会被特异性标记，因为在质粒DNA扩增的同时，染色体DNA会发生大量降解。
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