Oka H, Oka F, Ito Y
Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.
J Chromatogr. 1989 Sep 22;479(1):53-60. doi: 10.1016/s0021-9673(01)83316-1.
A compact portable model of a high-speed counter-current chromatograph enables efficient analytical separations of microgram sample quantities within 10 min. A series of preliminary experiments was conducted to study retention of the stationary phase of various two-phase solvent systems in short coils with different helical diameters. Under the optimal experimental conditions, analytical capability of the apparatus was successfully demonstrated in separation of flavonoids from a crude sea buckthorn ethanol extract in a multilayer coil with a total capacity of 8 ml.
一种紧凑型便携式高速逆流色谱仪能够在10分钟内对微克级样品量进行高效分析分离。进行了一系列初步实验，以研究不同螺旋直径的短线圈中各种两相溶剂系统固定相的保留情况。在最佳实验条件下，该仪器在一个总容量为8毫升的多层线圈中成功地从沙棘粗乙醇提取物中分离出黄酮类化合物，展示了其分析能力。
