College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, People's Republic of China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Environ Pollut. 2023 Apr 15;323:121233. doi: 10.1016/j.envpol.2023.121233. Epub 2023 Feb 15.
Microplastics have been recognized as a widespread new pollutant in nature and have induced an increase in the occurrence of a variety of diseases in carp. An animal model of microplastic ingestion was successfully established in an aqueous environment. The gut microbiota was analysed using a metagenomic approach. The results showed a significant reduction in the relative abundances of Lactococcus garvieae, Bacteroides_paurosaccharolyticus, and Romboutsia_ilealis after PS-MPs treatment. The 16S Silva database was used to predict and analyse the known genes. Intestinal flora disorders related to infectious diseases, cancers, neurodegenerative diseases, endocrine and metabolic diseases, cardiovascular diseases, and other diseases were found. The intake of PS-MPs resulted in damage to carp intestinal tissue and apoptosis of intestinal epithelial cells. The levels of the inflammatory cytokines IL-1β, IL-6, and TNF-α were significantly increased with the intake of PS-MPs. The gene and protein levels of GRP78, Caspase-3, Caspase-7, Caspase-9, Caspase-12, PERK, IRE1, and ATF6 were further examined in PS group. The occurrence of ERS and apoptosis in carp intestines was confirmed. These results suggest that the accumulation of PS-MPs in the aquatic environment can disturb the carp gut microbiota and induce ERS, apoptosis, and inflammation in the intestinal tissue.
微塑料已被确认为自然界中广泛存在的新型污染物，并导致鲤鱼多种疾病的发生率增加。在水相环境中成功建立了微塑料摄入的动物模型。采用宏基因组学方法分析肠道微生物群。结果表明，PS-MPs 处理后，Lactococcus garvieae、Bacteroides_paurosaccharolyticus 和 Romboutsia_ilealis 的相对丰度显著降低。使用 16S Silva 数据库预测和分析已知基因。发现与传染病、癌症、神经退行性疾病、内分泌和代谢疾病、心血管疾病等相关的肠道菌群失调。PS-MPs 的摄入导致鲤鱼肠道组织损伤和肠上皮细胞凋亡。随着 PS-MPs 的摄入，炎症细胞因子 IL-1β、IL-6 和 TNF-α 的水平显著升高。进一步检测 PS 组中 GRP78、Caspase-3、Caspase-7、Caspase-9、Caspase-12、PERK、IRE1 和 ATF6 的基因和蛋白水平。证实了鲤鱼肠道中 ERS 和细胞凋亡的发生。这些结果表明，水生环境中 PS-MPs 的积累会干扰鲤鱼肠道微生物群，并诱导肠道组织发生 ERS、细胞凋亡和炎症。
