绘制 21 种内源性蛋白在啮齿动物神经元高尔基体中的定位图谱。

Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons.

机构信息

Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands.

Functional Genomics, Department of Human Genetics, Center for Neurogenomics and Cognitive Research (CNCR), UMC Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands.

出版信息

Sci Rep. 2023 Feb 18;13(1):2871. doi: 10.1038/s41598-023-29998-8.

DOI:10.1038/s41598-023-29998-8

PMID:36806293

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9938882/

Abstract

The Golgi apparatus is the major sorting hub in the secretory pathway and particularly important for protein sorting in neurons. Knowledge about protein localization in Golgi compartments is largely based on work in cell lines. Here, we systematically compared protein localization of 21 endogenous proteins in the Golgi apparatus of mouse neurons using confocal microscopy and line scan analysis. We localized these proteins by measuring the distance relative to the canonical TGN marker TGN38. Based on this, proteins fell into three groups: upstream of, overlapping with or downstream of TGN38. Seven proteins showed complete overlap with TGN38, while proteins downstream of TGN38 were located at varying distances from TGN38. Proteins upstream of TGN38 were localized in between TGN38 and the cis-/medial Golgi markers Giantin and GM130. This localization was consistent with protein function. Our data provide an overview of the relative localization of endogenous proteins in the Golgi of primary mouse neurons.

摘要

高尔基体是分泌途径中的主要分拣中心，对于神经元中的蛋白质分拣尤为重要。关于高尔基体隔室中蛋白质定位的知识主要基于细胞系的工作。在这里，我们使用共聚焦显微镜和线扫描分析系统地比较了 21 种内源性蛋白质在小鼠神经元中的高尔基体中的定位。我们通过测量相对于规范的 TGN 标志物 TGN38 的距离来定位这些蛋白质。基于此，蛋白质分为三组：TGN38 的上游、重叠或下游。七种蛋白质与 TGN38 完全重叠，而 TGN38 下游的蛋白质与 TGN38 的距离不同。TGN38 上游的蛋白质定位于 TGN38 和顺面/中间高尔基体标志物 Giantin 和 GM130 之间。这种定位与蛋白质功能一致。我们的数据提供了初级小鼠神经元高尔基体中内源性蛋白质相对定位的概述。