Zhu Chenxu, Wang Zhaoning, Ren Bing
Ludwig Institute for Cancer Research, La Jolla, CA, USA.
Department of Cellular and Molecular Medicine, University of California San Diego, School of Medicine, La Jolla, CA, USA.
Methods Mol Biol. 2023;2611:155-185. doi: 10.1007/978-1-0716-2899-7_10.
Simultaneous detection of chromatin accessibility and transcription from the same cells promises to greatly facilitate the dissection of cell-type-specific gene regulatory programs in complex tissues. Paired-seq enables joint analysis of open chromatin and nuclear transcriptome from up to a million cells in parallel. It achieves ultra-high-throughput single-cell multiomics with the use of a combinatorial barcoding strategy involving sequential ligation of multiplexed DNA barcodes to chromatin DNA fragments and reverse transcription products, followed by high-throughput DNA sequencing of the resulting DNA libraries and deconvolution of single-cell multiomic maps based on cell-specific barcodes.
同时检测同一细胞中的染色质可及性和转录有望极大地促进对复杂组织中细胞类型特异性基因调控程序的剖析。配对测序（Paired-seq）能够并行联合分析多达一百万个细胞的开放染色质和核转录组。它通过一种组合条形码策略实现了超高通量单细胞多组学分析，该策略包括将多重DNA条形码顺序连接到染色质DNA片段和逆转录产物上，随后对所得DNA文库进行高通量DNA测序，并基于细胞特异性条形码对单细胞多组学图谱进行解卷积分析。
