Biophotonics Applied to Health Science, Nove de Julho University, Sao Paulo, SP, Brazil.
Cardiology Division, Department of Medicine, Federal University of Sao Paulo, São Paulo, SP, Brazil.
Photochem Photobiol. 2023 Nov-Dec;99(6):1420-1428. doi: 10.1111/php.13794. Epub 2023 Mar 5.
This study investigated the influence of red light-emitting diodes (LED, 630 nm) on different irradiation parameters and the number of applications on mesenchymal stem cells derived from adipose tissue (AdMSCs) metabolism and paracrine factors. The AdMSCs were irradiated with a LEDbox device (output power: 2452.5 mW; laser beam: 163.5 cm ; irradiance: 15 mW cm ) using radiant exposures of 0.5, 2, and 4 J cm , respectively. AdMSCs were irradiated once or every 48 h up to three irradiations. All molecular analyses were performed 24 h after the last irradiation. LED did not induce changes in cell count, DNA damage, and oxidative stress. A significant repercussion of the LED has been noticed after three irradiations with 4 J cm . AdMSCs had higher levels of IL-6, IGF-1, and NOx index. A higher ATP content and MMT/Resazurin assay were identified in AdMSCs irradiated three times with 4 J cm . Mitochondrial basal respiration, maximal respiration and proton leak under metabolic stress were reduced by 0.5 and 2 J cm irradiations. These data showed that three LED irradiations with 4 J cm may be a suitable parameter for future AdMSCs therapy because of its improved metabolic activity, ATP content, and IL-6, IGF-1, and nitric oxide secretion.
本研究探讨了红光发光二极管（LED，630nm）对不同辐照参数和应用次数对脂肪来源间充质干细胞（AdMSCs）代谢和旁分泌因子的影响。使用 LEDbox 设备（输出功率：2452.5mW；激光束：163.5cm；辐照度：15mW/cm）对 AdMSCs 进行辐照，辐照剂量分别为 0.5、2 和 4J/cm。AdMSCs 分别进行一次或每 48h 一次的辐照，最多进行三次辐照。所有分子分析均在最后一次辐照后 24h 进行。LED 未引起细胞计数、DNA 损伤和氧化应激的变化。在进行 4J/cm 三次辐照后，LED 产生了显著的影响。AdMSCs 的 IL-6、IGF-1 和 NOx 指数水平较高。在 4J/cm 辐照三次的 AdMSCs 中，检测到较高的 ATP 含量和 MMT/Resazurin 检测值。在代谢应激下，线粒体基础呼吸、最大呼吸和质子漏减少了 0.5 和 2J/cm 照射。这些数据表明，4J/cm 三次 LED 辐照可能是未来 AdMSCs 治疗的合适参数，因为它提高了代谢活性、ATP 含量以及 IL-6、IGF-1 和一氧化氮的分泌。
