Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D 'Alcontres, 31, 98166, Messina, Italy.
Sci Rep. 2023 Feb 17;13(1):2849. doi: 10.1038/s41598-023-28957-7.
Bacterial vaginosis (BV) is a common vaginal dysbiosis characterized by a malodorous discharge and irritation. The imbalance of the vaginal microbiota plays a key role in the development of BV. It has been demonstrated that Gardnerella vaginalis (GV), a facultative anaerobic bacillus, is involved in BV. Due to the rising number of antimicrobial-resistant species, recurrence of BV is becoming more frequent in women; thus, alternative treatments to antibiotics are needed. Natural substances have recently shown a great efficacy for the treatment of vaginal dysbiosis. Thus, this study aimed to investigate the beneficial effect of a product containing pea protein (PP), grape seed extract (GS) and lactic acid (LA) in an in vivo model of Gardnerella vaginalis-induced vaginosis by intravaginal administration of GV suspension (1 × 10 CFU/20 µL saline). Our results demonstrated that the product containing PP, GS and LA significantly reduced GV proliferation. More specifically, it significantly preserved tissue architecture and reduced neutrophil infiltration, inflammatory markers and sialidase activity when used both as a pre- or a post-treatment. Moreover, the product displayed strong bioadhesive properties. Therefore, our data suggested that the product containing PP, GS and LA could be used as alternative preventive or curative treatment for the management of BV.
细菌性阴道病（BV）是一种常见的阴道微生态失调症，其特征为有异味分泌物和刺激感。阴道微生物群的失衡在 BV 的发展中起着关键作用。已经证明，兼性厌氧菌加德纳菌（GV）参与了 BV 的发生。由于抗菌药物耐药性物种的数量不断增加，BV 在女性中的复发越来越频繁；因此，需要替代抗生素的治疗方法。天然物质最近在治疗阴道微生态失调方面显示出了很好的疗效。因此，本研究旨在通过阴道内给予 GV 悬浮液（1×10 CFU/20 μL 生理盐水），在体内 GV 诱导的阴道病模型中，研究含有豌豆蛋白（PP）、葡萄籽提取物（GS）和乳酸（LA）的产品的有益作用。我们的研究结果表明，含有 PP、GS 和 LA 的产品可显著减少 GV 的增殖。更具体地说，它在作为预防或治疗药物时都可显著保持组织结构，并减少中性粒细胞浸润、炎症标志物和唾液酸酶活性。此外，该产品具有很强的生物黏附性。因此，我们的数据表明，含有 PP、GS 和 LA 的产品可作为预防或治疗 BV 的替代药物。
