Pu Zongjin, Chen Xiaonan, Dong Bangjian, Ma Ping, Li Xiaobo
Traditional Chinese medicine Genomics Laboratory, School of Pharmacy, Shanghai Jiao Tong University, Minhang, Shanghai, P. R. China.
J Sep Sci. 2023 Apr;46(8):e2200737. doi: 10.1002/jssc.202200737. Epub 2023 Mar 3.
Sijunzi Decoction is composed of Ginseng Radix et Rhizoma, Atractylodes Macrocephalae Rhizoma, Poria, and Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle, and it is a classic formula for treating spleen deficiency syndrome in Chinese medicine. Clarifying the active substances is an effective way to develop Traditional Chinese medicine and innovative medicines. Carbohydrates, proteins, amino acids, saponins, flavonoids, phenolic acids, and inorganic elements in the decoction were analyzed by multiple approaches. A molecular network was also used for visualizing the ingredients in Sijunzi Decoction, and representative components were also quantified. The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids, and 0.483% inorganic elements. Molecular network and quantitative analysis used to characterize the chemical composition of Sijunzi Decoction. The present study systematically characterized the constituents of Sijunzi Decoction, revealed the composition ratio of each type of constituent, and provided a reference for study on the substance basis of other Chinese medicine.
四君子汤由人参、白术、茯苓、炙甘草组成，是中医治疗脾虚证的经典方剂。明确其活性成分是发展中药及创新药物的有效途径。采用多种方法对该汤剂中的碳水化合物、蛋白质、氨基酸、皂苷、黄酮类、酚酸类及无机元素进行了分析。还利用分子网络对四君子汤中的成分进行可视化展示，并对代表性成分进行了定量分析。检测到的成分占四君子汤冻干粉的74.544%，其中粗多糖占41.751%，糖（聚合度1 - 2）占17.826%，总皂苷占8.181%，不溶性沉淀物占2.427%，游离氨基酸占2.154%，总黄酮占1.177%，总酚酸占0.546%，无机元素占0.483%。分子网络和定量分析用于表征四君子汤的化学成分。本研究系统地对四君子汤的成分进行了表征，揭示了各类成分的组成比例，为其他中药的物质基础研究提供了参考。
