Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Phytomedicine. 2010 Feb;17(2):100-7. doi: 10.1016/j.phymed.2009.10.012.
Gegen Qinlian dispensing granule, a favorite composite formula, is a combination of Radix Puerariae Lobatae, Radix Scutellariae, Rhizoma Coptidis and Radix et Rhizoma Glycyrrhizae Praeparata cum Melle. To develop a method to overall evaluate correlation between the formula and its four raw herbs, LC fingerprints of the formula and its raw herbs were developed and LC-DAD-MS was employed to identify the components in the formula fingerprint. According to the characteristic fragmentation behavior of known flavonoids, alkaloids and saponins isolated from the four raw herbs as well as retention time, UV and MS data of detected compounds, a total of 23 constituents in the formula fingerprint were structurally characterized. Chemical correlation between the formula and the four crude herbs was evaluated qualitatively and quantitatively by the developed LC fingerprints. The results showed that 8 components in the formula fingerprint were addressed to Radix Puerariae Lobatae, 11 to Radix Scutellariae, 7 to Rhizoma Coptidis, 3 to Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, and that the relative area ratios of the common peaks in the formula vary slightly in comparison with corresponding ratios in its crude herbs, demonstrating the chemical constituents in the formula have patterns similar to those in its crude herbs.
对于黄连解毒颗粒这一常用的复方制剂，它是由葛根、黄芩、黄连和炙甘草组成的。为了开发一种综合评价该方剂与其四味原料药相关性的方法，我们建立了该方剂及其四味原料药的 LC 指纹图谱，并采用 LC-DAD-MS 对该方剂指纹图谱中的成分进行了鉴定。根据从四味原料药中分离得到的已知黄酮类、生物碱类和皂苷类化合物的特征裂解行为，以及检测到的化合物的保留时间、紫外和质谱数据，共对该方剂指纹图谱中的 23 个成分进行了结构表征。采用所建立的 LC 指纹图谱，对该方剂与其四味原料药进行了定性和定量的化学相关性评价。结果表明，该方剂指纹图谱中的 8 个成分与葛根相对应，11 个成分与黄芩相对应，7 个成分与黄连相对应，3 个成分与炙甘草相对应，且该方剂指纹图谱中共有峰的相对面积比值与相应原料药的比值略有差异，这表明该方剂的化学成分与原料药的化学成分具有相似的模式。
