Misic Jelena, Milenkovic Dusanka
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Max Planck Institute for Biology of Ageing, Cologne, Germany.
Methods Mol Biol. 2023;2615:219-228. doi: 10.1007/978-1-0716-2922-2_16.
Mitochondria are eukaryotic organelles of endosymbiotic origin that contain their own genetic material, mitochondrial DNA (mtDNA), and dedicated systems for mtDNA maintenance and expression. MtDNA molecules encode a limited number of proteins that are nevertheless all essential subunits of the mitochondrial oxidative phosphorylation system. Here, we describe protocols to monitor DNA and RNA synthesis in intact, isolated mitochondria. These in organello synthesis protocols are valuable techniques for studying the mechanisms and regulation of mtDNA maintenance and expression.
线粒体是具有内共生起源的真核细胞器，含有自己的遗传物质——线粒体DNA（mtDNA），以及用于mtDNA维持和表达的专门系统。mtDNA分子编码数量有限的蛋白质，但这些蛋白质都是线粒体氧化磷酸化系统的必需亚基。在这里，我们描述了监测完整分离线粒体中DNA和RNA合成的实验方案。这些体外合成实验方案是研究mtDNA维持和表达机制及调控的有价值技术。
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