Enriquez J A, López-Pérez M J, Montoya J
Departamento de Bioquimica y Biologia Molecular y Celular, Facultad de Veterinaria, Universidad de Zaragoza, Spain.
FEBS Lett. 1991 Mar 11;280(1):32-6. doi: 10.1016/0014-5793(91)80197-b.
Isolated rat brain mitochondria, when incubated in the presence of [alpha-32P]UTP in an appropriate incubation buffer, in which the energy requirements are provided by exogenous ADP in the presence of an oxidizable substrate, are able to support mitochondrial DNA transcription and RNA processing in a way faithfully resembling the in vivo process. Furthermore, we have strikingly found a saturation of the synthesis of mature 16 S and 12 S rRNA under conditions in which their RNA precursor as well as the mature mRNAs continue being synthesized. This suggests that synthesis of mature rRNAs could be regulated at the level of processing of their precursor rather than at the level of transcription.
分离的大鼠脑线粒体，当在含有[α-32P]UTP的合适孵育缓冲液中孵育时，其中能量需求由可氧化底物存在下的外源ADP提供，能够以与体内过程极其相似的方式支持线粒体DNA转录和RNA加工。此外，我们惊人地发现在其RNA前体以及成熟mRNA继续合成的条件下，成熟16S和12S rRNA的合成出现饱和。这表明成熟rRNA的合成可能在其前体加工水平而非转录水平受到调控。
