Dr. Buddolla's Institute of Life Sciences, Tirupati, 517503, India.
Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, 517 502, India.
Curr Microbiol. 2023 Feb 18;80(4):109. doi: 10.1007/s00284-023-03203-5.
In this work, the effects of probiotic supplementation on cisplatin toxicity in zebrafish (Danio rerio) were examined. For this study, adult female zebrafish were given cisplatin (G2), the probiotic, Bacillus megaterium (G3), and cisplatin+B. megaterium (G4) for 30 days, in addition to the control (G1). In order to investigate changes in antioxidative enzymes, ROS production, and histological changes after treatment, the intestines and ovaries were excised. The levels of lipid peroxidation, glutathione peroxidase, glutathione reductase, catalase, and superoxide dismutase were found to be significantly higher in the cisplatin group than in the control group in both the intestine and the ovaries. Administration of the probiotic and cisplatin effectively reversed this damage. Histopathological analyses showed that the cisplatin group had much more damage than the control group and that probiotic+cisplatin treatment significantly cured these damages. It opens the door to probiotics being combined with cancer-related drugs, which may be a more efficient approach for minimizing side effects. The underlying molecular mechanisms of probiotics must be further investigated.
本研究旨在探讨益生菌补充剂对斑马鱼（Danio rerio）顺铂毒性的影响。为此，将成年雌性斑马鱼分为四组：对照组（G1）、顺铂组（G2）、益生菌枯草芽孢杆菌（Bacillus megaterium）组（G3）和顺铂+益生菌组（G4），连续给药 30 天。为了研究治疗后抗氧化酶、ROS 产生和组织学变化，切除了肠道和卵巢。结果显示，与对照组相比，顺铂组在肠道和卵巢中的脂质过氧化水平、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、过氧化氢酶和超氧化物歧化酶的水平显著升高。益生菌和顺铂的联合使用可有效逆转这种损伤。组织病理学分析表明，顺铂组的损伤明显多于对照组，而益生菌+顺铂治疗可显著治愈这些损伤。这为将益生菌与癌症相关药物联合使用提供了可能，这可能是减少副作用的更有效方法。还需要进一步研究益生菌的潜在分子机制。
