Herold R, Christner P, Graver H, Rosenbloom J
J Dent Res. 1979 Mar;58(Spec Issue B):992-3. doi: 10.1177/00220345790580025601.
Antiserum was prepared to fetal bovine enamel matrix and was used to localize the amelogenins in developing bovine molars by immunofluorescent microscopy. Amelogenins could be identified to preameloblasts, secretory ameloblasts, stratum intermedium cells, and the newly deposited enamel matrix. Mature enamel matrix did not fluoresce except in a thin line along the DEJ and adjacent to the ameloblasts. Immature enamel matrix of murine and porcine teeth fluoresced when treated with antiserum to bovine enamel matrix. No other portions of tooth buds or other tissues reacted with the specific antiserum.
制备了针对胎牛牙釉质基质的抗血清，并通过免疫荧光显微镜用于定位发育中的牛磨牙中的釉原蛋白。釉原蛋白可在成釉细胞前体细胞、分泌性成釉细胞、中间层细胞和新沉积的牙釉质基质中被识别。成熟的牙釉质基质除了在沿牙本质釉质界的一条细线以及与成釉细胞相邻处外不发荧光。用针对牛牙釉质基质的抗血清处理时，小鼠和猪牙齿的未成熟牙釉质基质发荧光。牙胚的其他部分或其他组织均未与特异性抗血清发生反应。
