环状同源物 1 通过 miR-23b/SIRT1 轴抑制猪脂肪生成。

CircHOMER1 inhibits porcine adipogenesis via the miR-23b/SIRT1 axis.

机构信息

College of Animal Science, Shanxi Agricultural University, Taigu, China.

出版信息

FASEB J. 2023 Mar;37(3):e22828. doi: 10.1096/fj.202202048RR.

DOI:10.1096/fj.202202048RR

Abstract

Fat deposition is critical to pork quality. However, the mechanism of fat deposition remains to be elucidated. Circular RNAs (circRNAs) are ideal biomarkers and are involved in adipogenesis. Here, we investigated the effect and mechanism of circHOMER1 on porcine adipogenesis in vitro and in vivo. Western blotting, Oil red O staining, and HE staining were used to assess the function of circHOMER1 in adipogenesis. The results showed that circHOMER1 inhibited adipogenic differentiation of porcine preadipocytes and suppressed adipogenesis in mice. Dual-luciferase reporter gene, RIP, and pull-down assays demonstrated that miR-23b directly bound to circHOMER1 and the 3'-UTR of SIRT1. Rescue experiments further illustrated the regulatory relationship among circHOMER1, miR-23b, and SIRT1. Conclusively, we demonstrate that circHOMER1 plays an inhibitory role in porcine adipogenesis through miR-23b and SIRT1. The present study revealed the mechanism of porcine adipogenesis, which may be helpful to improve pork quality.

摘要

脂肪沉积对猪肉品质至关重要。然而，脂肪沉积的机制仍有待阐明。环状 RNA（circRNAs）是理想的生物标志物，参与脂肪生成。在这里，我们研究了 circHOMER1 对体外和体内猪脂肪生成的影响和机制。Western blot、油红 O 染色和 HE 染色用于评估 circHOMER1 在脂肪生成中的功能。结果表明，circHOMER1 抑制猪前体脂肪细胞的成脂分化，并抑制小鼠的脂肪生成。双荧光素酶报告基因、RIP 和下拉实验表明，miR-23b 直接结合 circHOMER1 和 SIRT1 的 3'-UTR。挽救实验进一步说明了 circHOMER1、miR-23b 和 SIRT1 之间的调节关系。总之，我们证明 circHOMER1 通过 miR-23b 和 SIRT1 抑制猪脂肪生成。本研究揭示了猪脂肪生成的机制，这可能有助于提高猪肉品质。