Kelly Robert G
Aix-Marseille Université, CNRS UMR 7288, IBDM, Marseille, France.
Dev Cell. 2023 Feb 27;58(4):257-266. doi: 10.1016/j.devcel.2023.01.010. Epub 2023 Feb 20.
Organogenesis requires the orchestrated development of multiple cell lineages that converge, interact, and specialize to generate coherent functional structures, exemplified by transformation of the cardiac crescent into a four-chambered heart. Cardiomyocytes originate from the first and second heart fields, which make different regional contributions to the definitive heart. In this review, a series of recent single-cell transcriptomic analyses, together with genetic tracing experiments, are discussed, providing a detailed panorama of the cardiac progenitor cell landscape. These studies reveal that first heart field cells originate in a juxtacardiac field adjacent to extraembryonic mesoderm and contribute to the ventrolateral side of the cardiac primordium. In contrast, second heart field cells are deployed dorsomedially from a multilineage-primed progenitor population via arterial and venous pole pathways. Refining our knowledge of the origin and developmental trajectories of cells that build the heart is essential to address outstanding challenges in cardiac biology and disease.
器官发生需要多个细胞谱系的协调发育，这些细胞谱系汇聚、相互作用并特化，以生成连贯的功能结构，心脏新月形转变为四腔心脏就是一个例证。心肌细胞起源于第一和第二心脏场，它们对最终心脏做出不同的区域贡献。在这篇综述中，讨论了一系列近期的单细胞转录组分析以及基因追踪实验，提供了心脏祖细胞景观的详细全景。这些研究表明，第一心脏场细胞起源于与胚外中胚层相邻的心脏旁场，并对心脏原基的腹外侧做出贡献。相比之下，第二心脏场细胞通过动脉和静脉极途径从多谱系预分化的祖细胞群体向背内侧分布。完善我们对构建心脏的细胞的起源和发育轨迹的认识，对于应对心脏生物学和疾病方面的突出挑战至关重要。
