Raugi G J, Olerud J E, Gown A M
Veterans Administration Medical Center, Seattle, WA 98108.
J Invest Dermatol. 1987 Dec;89(6):551-4. doi: 10.1111/1523-1747.ep12461198.
We examined partial thickness incised human wounds of 2, 3, 5, 7, and 14 days of age for the presence of thrombospondin by immunostaining and light microscopy. At 2, 3, 5, and 7 days after wounding, thrombospondin is present primarily at the cut edges of the lateral and deep margins of the wound. It appears to be cleared from these extracellular matrix sites, and is no longer detectable in those sites in most 14-day-old wounds. Thrombospondin staining is present, however, in increased amounts around the vascular channels within and adjacent to the 7- and 14-day wounds in increased amounts relative to vascular channels distant from the wound. Our observations are consistent with known in vitro data regarding the binding of thrombospondin to fibrin and components of the extracellular matrix, as well as with data showing that proliferating endothelial cells secrete more thrombospondin than quiescent endothelial cells. These data support the hypothesis that thrombospondin plays a role in the early organization of the extracellular matrix of wounds.
我们通过免疫染色和光学显微镜检查了2天、3天、5天、7天和14天龄的人类部分厚度切割伤口中血小板反应蛋白的存在情况。在受伤后2天、3天、5天和7天，血小板反应蛋白主要存在于伤口外侧和深部边缘的切口处。它似乎从这些细胞外基质部位清除，在大多数14天龄伤口的这些部位不再可检测到。然而，相对于远离伤口的血管通道，在7天和14天伤口内及相邻的血管通道周围，血小板反应蛋白染色量增加。我们的观察结果与关于血小板反应蛋白与纤维蛋白和细胞外基质成分结合的已知体外数据一致，也与显示增殖内皮细胞比静止内皮细胞分泌更多血小板反应蛋白的数据一致。这些数据支持了血小板反应蛋白在伤口细胞外基质早期组织中起作用的假说。
