Morita H
Department of Orthopedic Surgery, Akita University School of Medicine, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1987 Jun;61(6):765-73.
Fluorescein isothiocyanate-albumin conjugate was intravenously injected into the rat. The biceps femoris muscles were fixed by snap freezing with isopentane cooled to -150 degrees C. In histological sections infused capillaries were visualized under fluorescence microscopy. Perfused capillary density and number of capillaries per muscle fiber were determined at rest, during and after contraction of the muscle. Capillaries per muscle fiber ratios were 0.73 +/- 0.03 (mean +/- SE) at rest, 0.84 +/- 0.03 during weak contraction, 0.85 +/- 0.02 during strong contraction, and 0.85 +/- 0.02 in postcontraction hyperemia. During strong contraction, muscle blood flow measured by heated thermocouple technique showed a decrease and was not proportional to the increased capillary density. From these results it was assumed that additional factors such as increased velocity of the flow might play an important part in the increase in muscle blood flow during and after contraction.
将异硫氰酸荧光素 - 白蛋白共轭物静脉注射到大鼠体内。用冷却至 -150℃的异戊烷速冻固定股二头肌。在组织学切片中，通过荧光显微镜观察灌注的毛细血管。在肌肉静息、收缩期间和收缩后，测定灌注毛细血管密度和每根肌纤维的毛细血管数量。每根肌纤维的毛细血管比率在静息时为0.73±0.03（平均值±标准误），弱收缩时为0.84±0.03，强收缩时为0.85±0.02，收缩后充血时为0.85±0.02。在强收缩期间，用加热热电偶技术测量的肌肉血流量显示减少，且与增加的毛细血管密度不成比例。从这些结果推测，诸如血流速度增加等其他因素可能在收缩期间和收缩后肌肉血流量增加中起重要作用。
