Farrelly C A, Snyder G K
Department of EPO Biology, University of Colorado, Boulder 80309-0334.
Biotech Histochem. 1992 Sep;67(5):315-20. doi: 10.3109/10520299209110042.
We determined the effects of rapid freezing and perfusion fixation on fiber geometry and capillarity in rat skeletal muscle. Fiber areas were significantly decreased, and capillary densities significantly increased, in perfusion-fixed versus quick-frozen muscle. Significant differences in capillary-to-fiber ratios were not observed, suggesting that differences in fiber geometry, not the methods of quantifying capillaries, accounted for the differences in capillary density. We conclude that estimates of fiber geometry, capillarity, and diffusive gas conductances obtained from perfusion-fixed muscles are subject to significant error due to shrinkage.
我们确定了快速冷冻和灌注固定对大鼠骨骼肌纤维几何形态和毛细血管分布的影响。与速冻肌肉相比，灌注固定的肌肉中纤维面积显著减小，毛细血管密度显著增加。未观察到毛细血管与纤维比例的显著差异，这表明纤维几何形态的差异而非毛细血管量化方法导致了毛细血管密度的差异。我们得出结论，由于收缩作用，从灌注固定的肌肉中获得的纤维几何形态、毛细血管分布和扩散气体传导率的估计值存在显著误差。
