Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Brazil.
Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Brazil.
Physiol Plant. 2023 Mar;175(2):e13877. doi: 10.1111/ppl.13877.
Callose is a polymer deposited on the cell wall and is necessary for plant growth and development. Callose is synthesized by genes from the glucan synthase-like family (GSL) and dynamically responds to various types of stress. Callose can inhibit pathogenic infection, in the case of biotic stresses, and maintain cell turgor and stiffen the plant cell wall in abiotic stresses. Here, we report the identification of 23 GSL genes (GmGSL) in the soybean genome. We performed phylogenetic analyses, gene structure prediction, duplication patterns, and expression profiles on several RNA-Seq libraries. Our analyses show that WGD/Segmental duplication contributed to expanding this gene family in soybean. Next, we analyzed the callose responses in soybean under abiotic and biotic stresses. The data show that callose is induced by both osmotic stress and flagellin 22 (flg22) and is related to the activity of β-1,3-glucanases. By using RT-qPCR, we evaluated the expression of GSL genes during the treatment of soybean roots with mannitol and flg22. The GmGSL23 gene was upregulated in seedlings treated with osmotic stress or flg22, showing the essential role of this gene in the soybean defense response to pathogenic organisms and osmotic stress. Our results provide an important understanding of the role of callose deposition and regulation of GSL genes in response to osmotic stress and flg22 infection in soybean seedlings.
胼胝质是一种沉积在细胞壁上的聚合物，对植物的生长和发育是必需的。胼胝质由葡聚糖合酶样家族（GSL）的基因合成，并对各种类型的胁迫做出动态响应。在生物胁迫的情况下，胼胝质可以抑制病原感染，在非生物胁迫的情况下，它可以维持细胞膨压并使植物细胞壁变硬。在这里，我们报告了在大豆基因组中鉴定出 23 个 GSL 基因（GmGSL）。我们进行了系统发育分析、基因结构预测、重复模式和几个 RNA-Seq 文库的表达谱分析。我们的分析表明，WGD/片段重复有助于大豆中这个基因家族的扩张。接下来，我们分析了大豆在非生物和生物胁迫下的胼胝质反应。数据表明，胼胝质既被渗透胁迫又被鞭毛蛋白 22（flg22）诱导，与β-1,3-葡聚糖酶的活性有关。通过使用 RT-qPCR，我们评估了大豆根系在甘露醇和 flg22 处理过程中 GSL 基因的表达。GmGSL23 基因在受到渗透胁迫或 flg22 处理的幼苗中上调表达，表明该基因在大豆防御病原生物和渗透胁迫方面的重要作用。我们的研究结果为理解大豆幼苗中胼胝质沉积和 GSL 基因对渗透胁迫和 flg22 感染的调控作用提供了重要认识。
