Al-Rawi Natheer H, Rizvi Zuha, Mkadmi Sarra, Abu Kou Rawan, Elmabrouk Neibal, Alrashdan Mohammad S, Koippallil Gopalakrishnan Aghila Rani
Department of Oral & Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates.
Sharjah Institute of Medical Research, University of Sharjah, Sharjah, United Arab Emirates.
Eur J Dent. 2023 Oct;17(4):1215-1220. doi: 10.1055/s-0043-1761191. Epub 2023 Feb 22.
The aim of this study was to evaluate the expression of selected salivary oncomiRNAs among smokeless tobacco users and nonsmokers.
Twenty-five subjects with chronic smokeless tobacco habit (> 1 year) and 25 nonsmokers were selected for this study. MicroRNA was extracted from saliva samples using the miRNeasy Kit (Qiagen, Hilden, Germany). The forward primers used in the reactions include hsa-miR-21-5p, hsa-miR-146a-3p, hsa-miR-155-3p, and hsa-miR-199a-3p. Relative expression of miRNAs was calculated using the 2-ΔΔCt method. Fold change is calculated by raising 2 to the power of the negative ΔΔCT value.
Statistical analysis was carried out using GraphPad Prism 5 software. A -value less than 0.05 was considered statistically significant.
The four tested miRNAs were found overexpressed in saliva of subjects with smokeless tobacco habit when compared with saliva from nontobacco users. miR-21 expression was 3.74 ± 2.26 folds higher among subjects with smokeless tobacco habit compared to nontobacco users ( < 0.01). The expression for miR-146a (5.56 ± 8.3 folds; < 0.05), miR-155 (8.06 ± 23.4 folds; < 0.0001) and miR-199a (14.39 ± 30.3 folds; < 0.05) was significantly higher among subjects with smokeless tobacco habit.
Smokeless tobacco leads to salivary overexpression of the miRs 21, 146a, 155, and 199a. Monitoring the levels of these four oncomiRs may provide insight about the future development of oral squamous cell carcinoma, especially in patients with smokeless tobacco habits.
本研究旨在评估无烟烟草使用者和非吸烟者中特定唾液癌 miRNA 的表达情况。
本研究选取了 25 名有慢性无烟烟草使用习惯（超过 1 年）的受试者和 25 名非吸烟者。使用 miRNeasy 试剂盒（德国希尔德的 Qiagen 公司）从唾液样本中提取微小 RNA。反应中使用的正向引物包括 hsa-miR-21-5p、hsa-miR-146a-3p、hsa-miR-155-3p 和 hsa-miR-199a-3p。使用 2-ΔΔCt 方法计算 miRNA 的相对表达量。通过将 2 提升至负 ΔΔCT 值的幂来计算倍数变化。
使用 GraphPad Prism 5 软件进行统计分析。P 值小于 0.05 被认为具有统计学意义。
与非烟草使用者的唾液相比，发现这四种检测的 miRNA 在有无烟烟草使用习惯的受试者唾液中过表达。与非烟草使用者相比，有无烟烟草使用习惯的受试者中 miR-21 的表达高 3.74±2.26 倍（P<0.01）。miR-146a（5.56±8.3 倍；P<0.05）、miR-155（8.06±23.4 倍；P<0.0001）和 miR-199a（14.39±30.3 倍；P<0.05）在有无烟烟草使用习惯的受试者中的表达显著更高。
无烟烟草导致唾液中 miR-21、146a、155 和 199a 过表达。监测这四种癌 miRNA 的水平可能有助于深入了解口腔鳞状细胞癌的未来发展，尤其是在有无烟烟草使用习惯的患者中。
