Department of Dentistry, School of Dentistry, National Yang Ming Chiao Tung University, Taipei, 112304, Taiwan.
Department of Dentistry, School of Dentistry, National Yang Ming Chiao Tung University, Taipei, 112304, Taiwan; Department of Stomatology, Taipei Veterans Hospital, Taipei, 11221, Taiwan; Institute of Oral Tissue Engineering and Biomaterials, National Yang Ming Chiao Tung University, Taipei, 112304, Taiwan.
J Mech Behav Biomed Mater. 2023 Nov;147:106159. doi: 10.1016/j.jmbbm.2023.106159. Epub 2023 Sep 30.
Bioactive glass (BG) has been widely employed in the field of bone tissue engineering owing to its osteoconductive properties. These properties increase the stiffness and bioactivity of polymeric hydrogels, making them ideal for the repair, replacement, and regeneration of damaged bones. In this study, we investigated the effects of incorporating silanized 45S5 bioactive glass (Si-BG) into gelatin methacrylate (GelMA) hydrogel (GelMA/Si-BG) for potential bone tissue engineering. Our findings revealed that crosslinking GelMA with Si-BG had a striking increase in bioactivity with and without osteogenic induction of human mesenchymal stem cells (hMSCs) when compared to GelMA/BG hydrogels. Meanwhile, both GelMA/Si-BG and GelMA/BG hydrogels were able to maintain the cell viability of hMSC for up to 14 days. Additionally, GelMA/Si-BG hydrogels were shown to have a significantly higher compressive modulus than GelMA/BG hydrogels. This study has demonstrated the introduction of silanized 45S5 BG into GelMA hydrogel bioactivity and mechanical properties of GelMA hydrogels, exemplifying the potential application of silanization of BG in bone tissue engineering.
生物活性玻璃（BG）由于其骨诱导性能而被广泛应用于骨组织工程领域。这些特性增加了聚合物水凝胶的刚性和生物活性，使其成为修复、替代和再生受损骨骼的理想选择。在这项研究中，我们研究了将硅烷化 45S5 生物活性玻璃（Si-BG）掺入明胶甲基丙烯酸盐（GelMA）水凝胶（GelMA/Si-BG）中用于潜在骨组织工程的效果。我们的研究结果表明，与 GelMA/BG 水凝胶相比，交联 GelMA 与 Si-BG 具有显著提高的生物活性，无论是在有还是没有成骨诱导的情况下对人骨髓间充质干细胞（hMSC）的影响都是如此。同时，GelMA/Si-BG 和 GelMA/BG 水凝胶都能够维持 hMSC 的细胞活力长达 14 天。此外，GelMA/Si-BG 水凝胶的压缩模量明显高于 GelMA/BG 水凝胶。这项研究表明，将硅烷化 45S5 BG 引入 GelMA 水凝胶中可提高 GelMA 水凝胶的生物活性和机械性能，证明了 BG 硅烷化在骨组织工程中的潜在应用。
