Liao Tzu-Ying, King Peter C, Zhu Deming, Crawford Russell J, Ivanova Elena P, Thissen Helmut, Kingshott Peter
School of Science, Computing & Engineering Technologies, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia.
Australian Research Council (ARC) Training Centre in Surface Engineering for Advanced Materials (SEAM), Swinburne University of Technology, Hawthorn, Victoria 3122, Australia.
ACS Biomater Sci Eng. 2023 Mar 13;9(3):1402-1421. doi: 10.1021/acsbiomaterials.2c01506. Epub 2023 Feb 22.
A variant of the cold spray (CS) technique was applied for the functionalization of polymer-based materials such as polydimethylsiloxane (PDMS) to improve the extent of mammalian cell interactions with these substrates. This was demonstrated by the embedment of porous titanium (pTi) into PDMS substrates using a single-step CS technique. CS processing parameters such as gas pressure and temperature were optimized to achieve the mechanical interlocking of pTi in the compressed PDMS to fabricate a unique hierarchical morphology possessing micro-roughness. As evidenced by the preserved porous structure, the pTi particles did not undergo any significant plastic deformation upon impact with the polymer substrate. The thickness of the particle embedment layer was determined, by cross-sectional analysis, ranging from 120 μm to over 200 μm. The behavior of osteoblast-like cells MG63 coming into contact with the pTi-embedded PDMS was examined. The results showed that the pTi-embedded PDMS samples promoted 80-96% of cell adhesion and proliferation during the early stages of incubation. The low cytotoxicity of the pTi-embedded PDMS was confirmed, with cell viability of the MG63 cells being above 90%. Furthermore, the pTi-embedded PDMS facilitated the production of alkaline phosphatase and calcium deposition in the MG63 cells, as demonstrated by the higher amount of alkaline phosphatase (2.6 times) and calcium (10.6 times) on the pTi-embedded PDMS sample fabricated at 250 °C, 3 MPa. Overall, the work demonstrated that the CS process provided flexibility in the parameters used for the production of the modified PDMS substrates and is highly efficient for the fabrication of coated polymer products. The results obtained in this study suggest that a tailorable porous and rough architecture could be achieved that promoted osteoblast function, indicating that the method has promise in the design of titanium-polymer composite materials applied to biomaterials used in musculoskeletal applications.
冷喷涂（CS）技术的一种变体被应用于对基于聚合物的材料（如聚二甲基硅氧烷（PDMS））进行功能化处理，以提高哺乳动物细胞与这些底物的相互作用程度。这通过使用单步CS技术将多孔钛（pTi）嵌入PDMS底物中得到了证明。优化了诸如气体压力和温度等CS加工参数，以实现pTi在压缩PDMS中的机械互锁，从而制造出具有微粗糙度的独特分层形态。如保留的多孔结构所证明的，pTi颗粒在与聚合物底物碰撞时没有发生任何显著的塑性变形。通过横截面分析确定颗粒嵌入层的厚度，范围为120μm至超过200μm。研究了成骨样细胞MG63与嵌入pTi的PDMS接触时的行为。结果表明，在孵育早期，嵌入pTi的PDMS样品促进了80 - 96%的细胞粘附和增殖。证实了嵌入pTi的PDMS具有低细胞毒性，MG63细胞的细胞活力高于90%。此外，嵌入pTi的PDMS促进了MG63细胞中碱性磷酸酶的产生和钙沉积，如在250°C、3MPa下制造的嵌入pTi的PDMS样品上碱性磷酸酶（2.6倍）和钙（10.6倍）的含量更高所证明的。总体而言这项工作表明，CS工艺在用于生产改性PDMS底物的参数方面提供了灵活性，并且对于制造涂层聚合物产品非常高效。本研究获得的结果表明，可以实现一种可定制的多孔和粗糙结构，促进成骨细胞功能，这表明该方法在设计应用于肌肉骨骼应用生物材料的钛 - 聚合物复合材料方面具有前景。
