Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea.
Department of Dental Hygiene, College of Health Science, Gachon University, Incheon, 21936, South Korea.
Histochem Cell Biol. 2023 Jun;159(6):477-487. doi: 10.1007/s00418-023-02181-w. Epub 2023 Feb 22.
Mechanically activated factors are important in organogenesis, especially in the formation of secretory organs, such as salivary glands. Piezo-type mechanosensitive ion channel component 1 (Piezo1), although previously studied as a physical modulator of the mechanotransduction, was firstly evaluated on its developmental function in this study. The detailed localization and expression pattern of Piezo1 during mouse submandibular gland (SMG) development were analyzed using immunohistochemistry and RT-qPCR, respectively. The specific expression pattern of Piezo1 was examined in acinar-forming epithelial cells at embryonic day 14 (E14) and E16, which are important developmental stages for acinar cell differentiation. To understand the precise function of Piezo1 in SMG development, siRNA against Piezo1 (siPiezo1) was employed as a loss-of-function approach, during in vitro organ cultivation of SMG at E14 for the designated period. Alterations in the histomorphology and expression patterns of related signaling molecules, including Bmp2, Fgf4, Fgf10, Gli1, Gli3, Ptch1, Shh, and Tgfβ-3, were examined in acinar-forming cells after 1 and 2 days of cultivation. Particularly, altered localization patterns of differentiation-related signaling molecules including Aquaporin5, E-cadherin, Vimentin, and cytokeratins would suggest that Piezo1 modulates the early differentiation of acinar cells in SMGs by modulating the Shh signaling pathway.
机械激活因子在器官发生中很重要，特别是在分泌器官的形成中，如唾液腺。Piezo 型机械敏感离子通道成分 1（Piezo1），虽然以前被研究为机械转导的物理调节剂，但在本研究中首次评估了其在发育功能上的作用。通过免疫组织化学和 RT-qPCR 分别分析了 Piezo1 在小鼠下颌下腺（SMG）发育过程中的详细定位和表达模式。在 E14 和 E16 等重要的腺泡细胞分化发育阶段，检测了 Piezo1 在形成腺泡的上皮细胞中的特异性表达模式。为了了解 Piezo1 在 SMG 发育中的精确功能，在 E14 时通过体外器官培养，针对 Piezo1 的 siRNA（siPiezo1）作为一种失活功能的方法，在指定的时间段内被用于 SMG。在培养 1 和 2 天后，在形成腺泡的细胞中检测到与相关信号分子（包括 Bmp2、Fgf4、Fgf10、Gli1、Gli3、Ptch1、Shh 和 Tgfβ-3）的形态和表达模式的变化。特别是，分化相关信号分子（包括 Aquaporin5、E-cadherin、Vimentin 和细胞角蛋白）的定位模式的改变表明 Piezo1 通过调节 Shh 信号通路来调节 SMG 中腺泡细胞的早期分化。
