Developmental Roles of FUSE Binding Protein 1 () in Tooth Morphogenesis.

FUSE binding protein 1 (), a regulator of the c-Myc transcription factor and a DNA/RNA-binding protein, plays important roles in the regulation of gene transcription and cellular physiology. In this study, to reveal the precise developmental function of , we examined the detailed expression pattern and developmental function of during tooth morphogenesis by RT-qPCR, in situ hybridization, and knock-down study using in vitro organ cultivation methods. In embryogenesis, is obviously expressed in the enamel organ and condensed mesenchyme, known to be important for proper tooth formation. Knocking down at E14 for two days, showed the altered expression patterns of tooth development related signalling molecules, including and . In addition, transient knock-down of at E14 revealed changes in the localization patterns of c-Myc and cell proliferation in epithelium and mesenchyme, related with altered tooth morphogenesis. These results also showed the decreased amelogenin and dentin sialophosphoprotein expressions and disrupted enamel rod and interrod formation in one- and three-week renal transplanted teeth respectively. Thus, our results suggested that plays a modulating role during dentinogenesis and amelogenesis by regulating the expression pattern of signalling molecules to achieve the proper structural formation of hard tissue matrices and crown morphogenesis in mice molar development.