Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Sevilla, Spain.
Dpto. Fisiología Médica y Biofísica, Facultad de Medicina, Universidad de Sevilla, Sevilla, Spain.
Transl Stroke Res. 2024 Apr;15(2):378-387. doi: 10.1007/s12975-023-01136-6. Epub 2023 Feb 23.
Aneurysmal subarachnoid hemorrhage (aSAH) is a neurovascular disease produced by extravasation of blood to the subarachnoid space after rupture of the cerebral vessels. After bleeding, the immune response is activated. The role of peripheral blood mononuclear cells (PBMCs) in this response is a current subject of research. We have analysed the changes in PBMCs of patients with aSAH and their interaction with the endothelium, focusing on their adhesion and the expression of adhesion molecules. Using an in vitro adhesion assay, we observed that the adhesion of PBMCs of patients with aSAH is increased. Flow cytometry analysis shows that monocytes increased significantly in patients, especially in those who developed vasospasm (VSP). In aSAH patients, the expression of CD162, CD49d, CD62L and CD11a in T lymphocytes and of CD62L in monocytes increased. However, the expression of CD162, CD43, and CD11a decreased in monocytes. Furthermore, monocytes from patients who developed arteriographic VSP had lower expression of CD62L. In conclusion, our results confirm that after aSAH, monocyte count and adhesion of PBMCs increase, especially in patients with VSP, and that the expression of several adhesion molecules is altered. These observations can help predict VSP and to improve the treatment of this pathology.
颅内动脉瘤性蛛网膜下腔出血（aSAH）是一种脑血管疾病，是由于脑动脉破裂导致血液外渗到蛛网膜下腔引起的。出血后，免疫反应被激活。外周血单核细胞（PBMCs）在这种反应中的作用是当前研究的一个课题。我们分析了 aSAH 患者 PBMCs 的变化及其与内皮细胞的相互作用，重点关注其黏附和黏附分子的表达。通过体外黏附试验，我们观察到 aSAH 患者 PBMCs 的黏附增加。流式细胞术分析显示，患者单核细胞显著增加，尤其是发生血管痉挛（VSP）的患者。在 aSAH 患者中，T 淋巴细胞中 CD162、CD49d、CD62L 和 CD11a 的表达以及单核细胞中 CD62L 的表达增加。然而，单核细胞中 CD162、CD43 和 CD11a 的表达减少。此外，发生血管造影 VSP 的患者的单核细胞 CD62L 表达较低。总之，我们的结果证实，aSAH 后，单核细胞计数和 PBMCs 的黏附增加，特别是在发生 VSP 的患者中，并且几种黏附分子的表达发生改变。这些观察结果可以帮助预测 VSP，并改善对这种病理的治疗。
