Shadmehr Elham, Davoudi Amin, Damoon Sarmast Nima, Saatchi Masoud
Preventive Restorative Dental Sciences Department, Endodontics Division, University of California San Francisco, San Francisco, USA.
Department of Prosthodontics, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran.
Iran Endod J. 2019 Summer;14(3):178-184. doi: 10.22037/iej.v14i3.22451.
Calcium hypochlorite (CH) has been recently suggested as an endodontic irrigant. The aim of this investigation was to evaluate the antimicrobial efficacy of CH compared to sodium hypochlorite (NaOCl) and chlorhexidine (CHX) against multispecies biofilm in surface and deep dentinal tubules.
Minimal inhibitory concentration (MIC) of irrigant agents was assessed using a microdilution method. One hundred and twenty of human maxillary incisor teeth were prepared and infected with suspension of , and in an anaerobic jar for 7 days. Depending on irrigation solutions, specimens were divided into 4 groups (=30); group 1: 2% CHX, group 2: 5.25% sodium hypochlorite, group 3: 5% calcium hypochlorite, group 4: positive control (normal saline (NS)). Fifteen remained specimens were used as negative control. Surviving bacteria were sampled before (S1) and after irrigation from surface (S2) and deep (S3) dentin. The medium turbidity was visualized with spectrophotometry. Data were analyzed using analysis of variance followed by Tukey post hoc test (=0.05).
The MIC of CH against , and was 25, 8 and 7.5 µg/mL respectively. There were no significant differences in S1 among the test groups. Moreover, 2% CHX and 5% CH had significantly lower medium turbidity at both S2 and S3, in comparison with 5.25% NaOCl (=0.018 and 0.031, respectively). But there were no significant differences between 2% CHX and 5% CH at both S2 and S3 (=0.862 and 0.978, respectively).
Under the conditions of this study, 5% CH and 2% CHX are more effective than 5.25% NaOCl in the reduction of mixed-culture biofilm
最近有人提出次氯酸钙（CH）可作为一种根管冲洗剂。本研究的目的是评估CH与次氯酸钠（NaOCl）和氯己定（CHX）相比，对表层和深层牙本质小管中多菌种生物膜的抗菌效果。
采用微量稀释法评估冲洗剂的最低抑菌浓度（MIC）。制备120颗人上颌切牙，并在厌氧罐中用、和的悬液感染7天。根据冲洗液的不同，将标本分为4组（每组30个）；第1组：2% CHX，第2组：5.25%次氯酸钠，第3组：5%次氯酸钙，第4组：阳性对照（生理盐水（NS））。另外15个留存标本用作阴性对照。在冲洗前（S1）以及从表层（S2）和深层（S3）牙本质冲洗后对存活细菌进行采样。用分光光度法观察培养基的浊度。采用方差分析，然后进行Tukey事后检验（=0.05）对数据进行分析。
CH对、和的MIC分别为25、8和7.5μg/mL。各试验组在S1时无显著差异。此外，与5.25% NaOCl相比，2% CHX和5% CH在S2和S3时的培养基浊度均显著更低（分别为=0.018和0.031）。但在S2和S3时，2% CHX与5% CH之间均无显著差异（分别为=0.862和0.978）。
在本研究条件下，5% CH和2% CHX在减少混合培养生物膜方面比5.25% NaOCl更有效。
