Favaro L, Campanaro S, Fugaban J I I, Treu L, Jung E S, d'Ovidio L, de Oliveira D P, Liong M-T, Ivanova I V, Todorov S D
Department of Agronomy Food Natural Resources Animals and Environment (DAFNAE), Università degli Studi di Padova, Agripolis, Viale dell'Università 16, 35020 Legnaro, Italy.
Department of Biology, Università degli Studi di Padova, Via U. Bassi 58/b, 35121 Padova, Italy.
Benef Microbes. 2023 Mar 14;14(1):57-72. doi: 10.3920/BM2022.0067. Epub 2023 Feb 23.
Bacteriocins produced by lactic acid bacteria are proteinaceous antibacterial metabolites that normally exhibit bactericidal or bacteriostatic activity against genetically closely related bacteria. In this work, the bacteriocinogenic potential of strain ST58, isolated from oral cavity of a healthy volunteer was evaluated. To better understand the biological role of this strain, its technological and safety traits were deeply investigated through a combined approach considering physiological, metabolomic and genomic properties. Three out of 14 colonies generating inhibition zones were confirmed to be bacteriocin producers and, according to repPCR and RAPD-PCR, differentiation assays, and 16S rRNA sequencing it was confirmed to be replicates of the same strain, identified as , named ST58. Based on multiple isolation of the same strain ( ST58) over the 26 weeks in screening process for the potential bacteriocinogenic strains from the oral cavity of the same volunteer, strain ST58 can be considered a persistent component of oral cavity microbiota. Genomic analysis of ST58 revealed the presence of operons encoding for bacteriocins pediocin PA-1 and penocin A. The produced bacteriocin(s) inhibited the growth of spp. and some spp. used to determine the activity spectrum. The highest levels of production (6400 AU/ml) were recorded against strains after 24 h of incubation and the antimicrobial activity was inhibited after treatment of the cell-free supernatants with proteolytic enzymes. Noteworthy, ST58 also presented antifungal activity and key metabolites potentially involved in these properties were identified. Overall, this strain can be of great biotechnological interest towards the development of effective bio-preservation cultures as well as potential health promoting microbes.
乳酸菌产生的细菌素是蛋白质类抗菌代谢产物，通常对遗传关系密切的细菌表现出杀菌或抑菌活性。在这项工作中，对从一名健康志愿者口腔中分离出的ST58菌株的细菌素产生潜力进行了评估。为了更好地理解该菌株的生物学作用，通过综合考虑生理、代谢组学和基因组特性的方法，对其技术和安全特性进行了深入研究。在14个产生抑菌圈的菌落中，有3个被确认为细菌素产生菌，根据repPCR和RAPD-PCR、鉴别分析以及16S rRNA测序，证实它们是同一菌株的重复菌株，鉴定为，命名为ST58。基于在对同一志愿者口腔中潜在细菌素产生菌株的筛选过程中，在26周内多次分离到同一菌株（ST58），ST58菌株可被视为口腔微生物群的一个持久组成部分。对ST58的基因组分析揭示了编码细菌素pediocin PA-1和penocin A的操纵子的存在。所产生的细菌素抑制了用于确定活性谱的某些菌株和一些菌株的生长。在培养24小时后，对菌株的产量达到最高水平（6400 AU/ml），并且在用蛋白水解酶处理无细胞上清液后，抗菌活性受到抑制。值得注意的是，ST58还表现出抗真菌活性，并鉴定出了可能参与这些特性的关键代谢产物。总体而言，该菌株对于开发有效的生物保鲜培养物以及潜在的促进健康的微生物具有很大的生物技术价值。
