Wang Haiyan, Lin Yanting, Liu Qingsong, Zhou An, Bian Huixi, Zhang Wencheng, Hui Ailing, Wu Zeyu
Engineering Research Center of Bio-Process of Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230601, China.
The Experimental Research Center, Anhui University of Chinese Medicine, Hefei, 230038, China.
Curr Res Food Sci. 2023 Feb 4;6:100455. doi: 10.1016/j.crfs.2023.100455. eCollection 2023.
The main purpose of this study was to compare the antioxidant and anticancer activities of lycopene samples with different ratios of Z-isomers. Lycopene samples containing 5%, 30%, and 55% Z-isomers were successfully prepared by using thermal treatment combined with anti-solvent crystallization. The bio-accessibility of lycopene was estimated by the determination of partition factor (PF) and the results showed that lycopene with 55% Z-isomers possessed the highest bio-accessibility. Moreover, DPPH and ABTS assays suggested that the antioxidant activity of lycopene increased with the Z-isomers content from 5% to 55%. However, lycopene inhibited the survival of human hepatocellular carcinoma cells (HepG2) in a dose and time-dependent manner. The highest inhibition of HepG2 cell lines was achieved by 55% Z-ratio of lycopene. The cell viability was 22.54% at 20 μg/mL after incubating for 24 h, the number of cells was significantly reduced and the morphology was shrunk. Furthermore, molecular docking was introduced to compare the binding ability between different lycopene isomers with Scavenger Receptor class B type I (SR-BI), and the results revealed that the affinity of (all-E)-lycopene with SR-BI was lower compared to 5Z-lycopene and 13Z-lycopene, providing the reasons for different bioavailability of the above-mentioned lycopene isomers. All the above results demonstrated that Z-isomers-rich lycopene could enhance bio-accessibility and biological functionality.
本研究的主要目的是比较不同Z-异构体比例的番茄红素样品的抗氧化和抗癌活性。通过热处理结合反溶剂结晶法成功制备了Z-异构体含量分别为5%、30%和55%的番茄红素样品。通过测定分配系数（PF）评估番茄红素的生物可及性，结果表明Z-异构体含量为55%的番茄红素具有最高的生物可及性。此外，DPPH和ABTS分析表明，番茄红素的抗氧化活性随着Z-异构体含量从5%增加到55%而增强。然而，番茄红素以剂量和时间依赖性方式抑制人肝癌细胞（HepG2）的存活。Z-异构体比例为55%的番茄红素对HepG2细胞系的抑制作用最强。孵育24小时后，在20μg/mL浓度下细胞活力为22.54%，细胞数量显著减少，形态萎缩。此外，引入分子对接比较不同番茄红素异构体与I型清道夫受体B类（SR-BI）的结合能力，结果表明与5Z-番茄红素和13Z-番茄红素相比，（全-E）-番茄红素与SR-BI的亲和力较低，这为上述番茄红素异构体生物利用度不同提供了原因。上述所有结果表明，富含Z-异构体的番茄红素可以提高生物可及性和生物学功能。
