Mahdavi Rabee, Talebpour Zahra
Department of Analytical Chemistry, Faculty of Chemistry, Alzahra University, Vanak, Tehran, Iran.
Analytical and Bioanalytical Research Centre, Alzahra University, Vanak, Tehran, Iran.
Trends Analyt Chem. 2023 Mar;160:116964. doi: 10.1016/j.trac.2023.116964. Epub 2023 Feb 13.
Since the outbreak of the COVID-19 pandemic, the use of antiviral and other available drugs has been considered to combat or reduce the clinical symptoms of patients. In this regard, it would be necessary to choose sensitive and selective analytical techniques for pharmacokinetic and pharmacodynamic studies, monitoring of drug concentration in biological fluids, and determination of the most appropriate dose to achieve the desired effect on the disease. In the present study, the analytical techniques based on spectroscopy and chromatography with different detectors for diagnosis and determination of candidate drugs in the treatment of COVID-19 in human biological fluids are reviewed during the period 2015-2022. Moreover, various sample preparation and extraction techniques, are being used for this purpose, such as protein precipitation (PP), solid-phase extraction (SPE), liquid-liquid extraction (LLE), and QuEChERS (quick, easy, cheap, effective, rugged, and safe) are investigated.
自新冠疫情爆发以来，使用抗病毒药物及其他可用药物被认为可对抗或减轻患者的临床症状。在这方面，有必要选择灵敏且具选择性的分析技术用于药代动力学和药效学研究、监测生物流体中的药物浓度以及确定最适宜的剂量以达到对疾病的预期疗效。在本研究中，对2015年至2022年期间基于光谱学和带有不同检测器的色谱法的分析技术进行了综述，这些技术用于诊断和测定人体生物流体中治疗新冠的候选药物。此外，还研究了为此目的而使用的各种样品制备和提取技术，如蛋白沉淀法（PP）、固相萃取法（SPE）、液液萃取法（LLE）以及QuEChERS法（快速、简便、廉价、高效、耐用且安全）。
