Hachef Afifa, Bourguiba Hédia, Cherif Emira, Ivorra Sarah, Terral Jean-Frédéric, Zehdi-Azouzi Salwa
University of Tunis El Manar, Faculté des Sciences de Tunis, Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Campus Universitaire, 2092 El Manar, Tunis, Tunisia.
ISEM, Univ Montpellier, CNRS, IRD, Montpellier, France.
Saudi J Biol Sci. 2023 Mar;30(3):103574. doi: 10.1016/j.sjbs.2023.103574. Epub 2023 Jan 27.
Date palm ( L.) like other crop species in the arid Mediterranean region is being threatened by genetic erosion and climate change. Therefore, the understanding and assessment of the diversity extent of this species is a primary requisite for preserving these crop resources. This study was designed to quantify the potential of Tunisian male date palms using a set of agro-morphological characteristics i.e. flowering traits, inflorescence morphology and pollen quality. The flowering time traits exhibited a trend of precocious phenotype at emergence spathe trait and the dominance of the full-season phenotype at the opening date. At inflorescence morphology, all observed traits expressed wide ranges which reflected the broad variability of the evaluated male genotypes. Significant difference was recorded for the majority of the examined traits with a high significant variation in the tree quantitative traits: Spathe Total Length, Spathe Maximum Width and Length to the brunched part. Pollen viability ranged from 51.10% to 98.75% while the germination rate was between 0.90% and 70.50%. Our phenotypic investigation has allowed the identification of males with desirable agronomic traits which have been genotyped using 18 nuclear SSR markers and a chloroplast minisatellite for preservation and effective utilization purposes.
海枣(Phoenix dactylifera L.)与干旱地中海地区的其他作物品种一样,正受到基因侵蚀和气候变化的威胁。因此,了解和评估该物种的多样性程度是保护这些作物资源的首要条件。本研究旨在利用一组农业形态特征,即开花性状、花序形态和花粉质量,量化突尼斯雄性海枣的潜力。开花时间性状在佛焰苞出现性状上表现出早熟表型趋势,在开放日期表现出全季表型的优势。在花序形态方面,所有观察到的性状表现出广泛的范围,这反映了所评估雄性基因型的广泛变异性。大多数检测性状记录到显著差异,树木数量性状存在高度显著变异:佛焰苞总长度、佛焰苞最大宽度和至分枝部分的长度。花粉活力范围为51.10%至98.75%,而发芽率在0.90%至70.50%之间。我们的表型研究已鉴定出具有理想农艺性状的雄性植株,这些植株已使用18个核SSR标记和一个叶绿体微卫星进行基因分型,以用于保存和有效利用目的。