Kalitin Nikolay, Dudina Galina, Kostritsa Natalia, Sivirinova Anastasiya, Karamysheva Aida
Laboratory of Tumor Cell Genetics, N.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, Russia.
Department of Oncohematology, A.S. Loginov Moscow Clinical Scientific Center, 111123 Moscow, Russia.
Oncol Lett. 2023 Jan 26;25(3):95. doi: 10.3892/ol.2023.13681. eCollection 2023 Mar.
Vascular endothelial growth factors (VEGFs) are angiogenic factors playing a key role in tumor development. VEGFs are produced by different normal and tumor cells, including platelets, lymphocytes and mononuclear cells of peripheral blood. ( and ) and ( and ) gene expression was studied in patients with myelodysplastic syndrome (MDS) to evaluate the possible prognostic role of the expression of these genes. Gene expression levels were determined using peripheral blood samples of 51 patients with MDS and 15 healthy volunteers by quantitative PCR. Expression of all and genes was elevated in patients with MDS compared with healthy volunteers. No association of expression with the hemoglobin content in peripheral blood was found. The analyses of gene expression in patients with MDS stratified by risk groups according to the International Prognostic Scoring System showed progressive augmentation of gene expression from low to high-risk groups and and expression from intermediate-1 to high-risk groups. The statistically significant difference in survival time of patients with high and low levels of expression was revealed. / expression may be important for risk evaluation of patients with MDS.
血管内皮生长因子（VEGFs）是在肿瘤发展中起关键作用的血管生成因子。VEGFs由不同的正常细胞和肿瘤细胞产生，包括血小板、淋巴细胞和外周血单核细胞。对骨髓增生异常综合征（MDS）患者的（和）以及（和）基因表达进行了研究，以评估这些基因表达可能的预后作用。通过定量PCR使用51例MDS患者和15名健康志愿者的外周血样本测定基因表达水平。与健康志愿者相比，MDS患者中所有和基因的表达均升高。未发现表达与外周血血红蛋白含量之间存在关联。根据国际预后评分系统按风险组分层的MDS患者的基因表达分析显示，基因表达从低风险组到高风险组逐渐增加，而和表达从中间-1风险组到高风险组逐渐增加。揭示了表达水平高低的患者生存时间的统计学显著差异。/表达可能对MDS患者的风险评估很重要。
