Hirase Shotaro, Sekino Masashi, Hara Motoyuki, Kikuchi Kiyoshi
Fisheries Laboratory, Graduate School of Agricultural and Life Sciences The University of Tokyo Shizuoka Japan.
Bioinformatics and Biosciences Division, Fisheries Resources Institute Japan Fisheries Research and Education Agency Yokohama Japan.
Ecol Evol. 2023 Feb 17;13(2):e9816. doi: 10.1002/ece3.9816. eCollection 2023 Feb.
The genetic basis of speciation in free-spawning marine invertebrates is poorly understood. Although gene copy number variations (GCNVs) and nucleotide variations possibly trigger the speciation of these organisms, empirical evidence for such a hypothesis is limited. In this study, we searched for genomic signatures of GCNVs that may contribute to the speciation of Western Pacific abalone species. Whole-genome sequencing data suggested the existence of significant amounts of GCNVs in closely related abalones, and , in the early phase of speciation. In addition, the degree of interspecies genetic differentiation in the genes where GCNVs were estimated was higher than that in other genes, suggesting that nucleotide divergence also accumulates in the genes with GCNVs. GCNVs in some genes were also detected in other related abalone species, suggesting that these GCNVs are derived from both ancestral and de novo mutations. Our findings suggest that GCNVs have been accumulated in the early phase of free-spawning abalone speciation.
对于自由产卵的海洋无脊椎动物物种形成的遗传基础，我们了解甚少。尽管基因拷贝数变异（GCNV）和核苷酸变异可能触发这些生物的物种形成，但这一假设的实证证据有限。在本研究中，我们寻找了可能有助于西太平洋鲍鱼物种形成的GCNV基因组特征。全基因组测序数据表明，在亲缘关系密切的鲍鱼以及物种形成早期阶段，存在大量的GCNV。此外，估计存在GCNV的基因中的种间遗传分化程度高于其他基因，这表明核苷酸差异也在具有GCNV的基因中积累。在其他相关鲍鱼物种中也检测到一些基因中的GCNV，这表明这些GCNV源自祖先突变和新生突变。我们的研究结果表明，GCNV在自由产卵的鲍鱼物种形成早期就已积累。
