Evaluation of the and Gene changes in women with premature ovarian failure: A case-control study.

BACKGROUND

Premature ovarian failure (POF), is menopause occurring before the age of 40, affecting 1-3% of women worldwide. The risk of POF increases with altered immunological parameters such as and genes, which play a fundamental role in embryogenesis and cellular homeostasis.

OBJECTIVE

The study aimed to investigate the potential role of and genes in POF pathogenesis.

MATERIALS AND METHODS

In this case-control study, the polymorphisms of -670A/G and IVS2nt_124A/G apoptotic genes were analyzed in 51 Iranian women suffering from POF, and 61 healthy controls. Isolation of DNA was done using the salting-out method, and genotypic analysis was performed for all the subjects using the polymerase chain reaction-restriction fragment length polymorphism method.

RESULTS

Our results revealed that homozygous -670A/A and G/G, and heterozygous -670A/G are not significantly different between cases and controls (p = 0.99). Also, in different genotyping models of IVS2nt_124, polymorphisms were not related to POF risk (p = 0.23).

CONCLUSION

There is no statistical association between these polymorphisms and POF risk in women referred to genetic counseling clinics.