Akhavansales Zhima, Mosadeghmehrjardi Alimohammad, Reza Ashrafzadeh Hamid, Fakhteh Yavari Shadnaz, Taher Tahoori Mohammad, Bitaraf Sani Morteza, Mohammadi Mahnaz, Montazeri Fateme, Ghasemi Nasrin
Department of Immunology, Faculty of Medicine, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Int J Reprod Biomed. 2023 Jan 9;20(12):1007-1012. doi: 10.18502/ijrm.v20i12.12561. eCollection 2022 Dec.
Premature ovarian failure (POF), is menopause occurring before the age of 40, affecting 1-3% of women worldwide. The risk of POF increases with altered immunological parameters such as and genes, which play a fundamental role in embryogenesis and cellular homeostasis.
The study aimed to investigate the potential role of and genes in POF pathogenesis.
In this case-control study, the polymorphisms of -670A/G and IVS2nt_124A/G apoptotic genes were analyzed in 51 Iranian women suffering from POF, and 61 healthy controls. Isolation of DNA was done using the salting-out method, and genotypic analysis was performed for all the subjects using the polymerase chain reaction-restriction fragment length polymorphism method.
Our results revealed that homozygous -670A/A and G/G, and heterozygous -670A/G are not significantly different between cases and controls (p = 0.99). Also, in different genotyping models of IVS2nt_124, polymorphisms were not related to POF risk (p = 0.23).
There is no statistical association between these polymorphisms and POF risk in women referred to genetic counseling clinics.
卵巢早衰（POF）是指在40岁之前出现的绝经，影响全球1%-3%的女性。POF的风险会随着免疫参数的改变而增加，如 和 基因，这些基因在胚胎发育和细胞稳态中起着重要作用。
本研究旨在探讨 和 基因在POF发病机制中的潜在作用。
在这项病例对照研究中，对51名患有POF的伊朗女性和61名健康对照者分析了凋亡基因-670A/G和IVS2nt_124A/G的多态性。采用盐析法提取DNA，并使用聚合酶链反应-限制性片段长度多态性方法对所有受试者进行基因分型分析。
我们的结果显示，病例组和对照组之间纯合子-670A/A和G/G以及杂合子-670A/G没有显著差异（p = 0.99）。此外，在IVS2nt_124的不同基因分型模型中，多态性与POF风险无关（p = 0.23）。
在前往遗传咨询诊所的女性中，这些多态性与POF风险之间没有统计学关联。
